3.4.24.27: thermolysin
This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.
Word Map on EC 3.4.24.27
-
3.4.24.27
-
chymotrypsin
-
elastase
-
metalloprotease
-
subtilisin
-
staphylococcus
-
edman
-
pepsin
-
aureus
-
carboxypeptidase
-
endopeptidase
-
bromide
-
cyanogen
-
collagenase
-
proteinases
-
dipeptide
-
angiotensin
-
pronase
-
metalloproteinases
-
alpha-chymotrypsin
-
thermolytic
-
hydrolysates
-
metalloendopeptidase
-
stearothermophilus
-
endoproteinase
-
phosphoramidon
-
i-converting
-
enkephalinase
-
rhodopsin
-
2-macroglobulin
-
3.4.24.11
-
cell-binding
-
alcalase
-
ace-inhibitory
-
hexxh
-
dispase
-
aspartame
-
thiorphan
-
neprilysin
-
s-carboxymethylated
-
half-cystine
-
astacin
-
synthesis
-
industry
-
nutrition
-
food industry
-
diagnostics
-
medicine
-
analysis
- 3.4.24.27
- chymotrypsin
- elastase
- metalloprotease
- subtilisin
- staphylococcus
-
edman
- pepsin
- aureus
- carboxypeptidase
- endopeptidase
- bromide
-
cyanogen
- collagenase
- proteinases
- dipeptide
- angiotensin
- pronase
- metalloproteinases
- alpha-chymotrypsin
-
thermolytic
- hydrolysates
- metalloendopeptidase
- stearothermophilus
-
endoproteinase
- phosphoramidon
-
i-converting
- enkephalinase
- rhodopsin
-
2-macroglobulin
-
3.4.24.11
-
cell-binding
- alcalase
-
ace-inhibitory
-
hexxh
-
dispase
- aspartame
- thiorphan
- neprilysin
-
s-carboxymethylated
-
half-cystine
- astacin
- synthesis
- industry
- nutrition
- food industry
- diagnostics
- medicine
- analysis
Reaction
preferential cleavage: -/-Leu > -/-Phe =
Synonyms
Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste
ECTree
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KCat KM Value
KCat KM Value on EC 3.4.24.27 - thermolysin
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13.1
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
73.5
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
444.3
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2
-
pH 7.4, 30°C
1.03
-
recombinant mutant L155A/I156V, pH 7.0, 37°C
2
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155A/I156N, pH 7.0, 37°C
3.67
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant wild-type enzyme, pH 7.0, 37°C
4
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155A, pH 7.0, 37°C
4
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S/I156N, pH 7.0, 37°C
12
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S/I156V, pH 7.0, 37°C
12.67
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S, pH 7.0, 37°C
9.6
-
recombinant mutant G117R, pH 7.5, 25°C
14.7
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant mutant G117K, pH 7.5, 25°C
18.8
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant wild-type enzyme, pH 7.5, 25°C
23.9
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant mutant G117E, pH 7.5, 25°C
6
-
mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
7.5
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
7.9
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
9.2
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
9.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
wild type enzyme, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
10
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
16.8
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
33.1
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
49.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
96.2
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
99
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
1.5
-
mutant enzyme F114A, at pH 7.5, at 25°C
9.3
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
wild type enzyme, at pH 7.5, at 25°C
11.7
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150A, at pH 7.5, at 25°C
13.7
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme N227H, at pH 7.5, at 25°C
16
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme I168A, at pH 7.5, at 25°C
23
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150W, at pH 7.5, at 25°C
27.6
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150E, at pH 7.5, at 25°C
11
purified recombinant mutant N116T, pH 7.5, 25°C
16
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116A, pH 7.5, 25°C
19
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116Q, pH 7.5, 25°C
20
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant wild-type enzyme, pH 7.5, 25°C
27
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116D, pH 7.5, 25°C
0.23
-
mutant enzyme S53D/L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.26
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.29
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.29
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
wild type enzyme, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.31
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.32
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.33
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D/L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.34
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.62
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme I168A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.79
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
1.82
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
2.04
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S/D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
2.1
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
2.49
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S/D150E/S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme I168A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.2
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D/L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.3
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D/L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.5
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.8
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
wild type enzyme, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.9
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
4
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
4
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
4.3
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S/D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6.6
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
9
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
11.7
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme L144S/D150E/S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
15
-
recombinant mutant G117R, pH 7.5, 25°C
16
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
-
recombinant mutant G117K, pH 7.5, 25°C
26
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
purified recombinant mutant N116T, pH 7.5, 25°C
31
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
purified recombinant mutant N116A, pH 7.5, 25°C
33
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
-
recombinant mutant G117E, pH 7.5, 25°C
37
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
purified recombinant mutant N116Q, pH 7.5, 25°C
37
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
purified recombinant wild-type enzyme, pH 7.5, 25°C
40
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
-
recombinant wild-type enzyme, pH 7.5, 25°C
117
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
purified recombinant mutant N116D, pH 7.5, 25°C