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3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

KCat KM Value

KCat KM Value on EC 3.4.24.27 - thermolysin

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.1
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
154.2
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2
-
pH 7.4, 30°C
73.5
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
444.3
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2
-
pH 7.4, 30°C
255.2
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2
-
pH 7.4, 30°C
60.3
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2
-
pH 7.5, 37°C
24.3
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2
-
pH 7.5, 37°C
1.03 - 12.67
N-benzyloxycarbonyl-Gly-L-Leu amide
9.6 - 23.9
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
6 - 99
N-carbobenzoxy-L-Asp-L-Phe methyl ester
1.5 - 27.6
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
11 - 27
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
0.23 - 11.7
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
15 - 117
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide