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3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

KI Value

KI Value on EC 3.4.24.27 - thermolysin

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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000000064
1-beta-D-arabinofuranosyl-N4-lauroylcytosine
-
25°C, pH 7.5
50 - 460
1-butanol
35
1-Pentanol
-
pH 7.5, 25°C
38 - 280
1-propanol
0.0593
2-(4-methylphenyl)-3-(1,3-thiazol-2-yl) quinazolin-4(3H)-one
-
at pH 7.0 and 25°C
0.57
2-(acetyloxy)-3-chlorobenzoic acid
in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C
40 - 370
2-butanol
0.315
2-ethyl-3-hydroxyquinazolin-4(3H)-one
in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C
0.00125
2-ethylquinazolin-4(3H)-one
-
at pH 7.0 and 25°C
2.06
2-hydroxy-N-(4-methyl-2-nitrophenyl)-3-nitrobenzamide
-
25°C, pH 7.5
41 - 380
2-methyl-1-propanol
1.6
2-phenylpropionyl-L-phenylalanine
-
pH 8.6, temperature not specified in the publication
0.000015
2-phenylpropionyl-Leu-Trp
-
pH 7.2, temperature not specified in the publication
0.000243
3-(isopropylideneamino)-2,2-dimethyl-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 25°C
0.0183
3-([(1E)-[4-(dimethylamino) phenyl]methylene]amino)-2-methylquinazolin-4(3H)-one
-
at pH 7.0 and 25°C
0.042
3-([(1E)-[4-(dimethylamino) phenyl]methylene]amino)-2-phenylquinazolin-4(3H)-one
-
at pH 7.0 and 25°C
10100
3-amino-2-(4-chlorophenyl)quinazolin-4(3H)-one
-
at pH 7.0 and 25°C
3.12
3-amino-2-(4-nitrophenyl)quinazolin-4(3H)-one
-
at pH 7.0 and 25°C
0.0379
3-amino-2-(trifluoromethyl) quinazolin-4(3H)-one
-
at pH 7.0 and 25°C
0.0549
3-amino-2-methylquinazolin-4(3H)-one
-
at pH 7.0 and 25°C
0.128
3-hydroxy-2-isopropylquinazolin-4(3H)-one
in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C
0.93
3-hydroxy-2-methylquinazolin-4(3H)-one
in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C
1.7
3-methylaspirin
in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C
0.0000115
3-phenyl-2-(trifluoromethyl) quinazolin-4(3H)-one
-
at pH 7.0 and 25°C
4
3-[[(1E)-(3-chlorophenyl)methylene]amino]-2-phenylquinazolin-4(3H)-one
-
at pH 7.0 and 25°C
4.81
5-bromo-N-(4-bromophenyl)-2-hydroxy-3-nitro-benzamide
-
25°C, pH 7.5
1.6
beta-phenylpropionyl-L-phenylalanine
-
pH 6.8, 25°C, enzyme contains Zn2+
0.000000068
carbobenzoxy-L-phenylalanine-phosphonamidate-L-leucyl-L-alanine
pH and temperature not specified in the publication
0.0016
CGS-28,106
pH 8.0, 37°C
7.63
dimethylformamide
-
pH 7.5, 25°C
7.7
DMSO
-
pH 7.5, 25°C
106
ethanimidic acid N-[4-oxo-2-phenyl-3(4H)-quinazolinyl]-ethyl ester
-
at pH 7.0 and 25°C
100
ethanol
-
pH 7.5, 25°C
0.0031 - 0.031
HACBO-Gly
0.25
Isopropanol
-
pH 7.5, 25°C
430
methanol
-
pH 7.5, 25°C
0.00005
N-(1-carboxy-3-phenyl-propyl)-L-leucyl-L-tryptophan
pH and temperature not specified in the publication
294
N-(2,3-dimethylphenyl)-2-hydroxy-3-nitro-benzamide
-
25°C, pH 7.5
161
N-(2,4-dimethylphenyl)-2-hydroxy-3-nitro-benzamide
-
25°C, pH 7.5
1.45
N-(2,5-dimethylphenyl)-2-hydroxy-3-nitrobenzamide
-
25°C, pH 7.5
697
N-(2-chloro-4-nitrophenyl)-2-hydroxy-3-nitro-benzamide
-
25°C, pH 7.5
0.047
N-(2-chloro-6-methylphenyl)-2-hydroxy-3-nitrobenzamide
-
25°C, pH 7.5
2.95
N-(2-ethylphenyl)-2-hydroxy-3-nitrobenzamide
-
25°C, pH 7.5
8.14
N-(5-chloro-2-methoxyphenyl)-2-hydroxy-3-nitro-benzamide
-
25°C, pH 7.5
0.008 - 0.37
N-Benzyloxycarbonyl-L-phenylalanine
0.007 - 0.21
N-benzyloxycarbonyl-L-tryptophan
0.18
n-Propanol
-
pH 7.5, 25°C
0.214
oxyquinoline
-
25°C, pH 7.5
0.000015
P-Leu-Trp
-
pH 7.2, 25°C, enzyme contains Zn2+
0.00003 - 0.0047
phosphoramidon
0.0008 - 0.16
Retrothiorphan
110
tert-amylalcohol
-
pH 7.5, 25°C
190
tert-butyl alcohol
-
pH 7.5, 25°C
0.0016 - 0.16
thiorphan
0.008 - 0.37
Z-L-phenylalanine
0.007 - 0.087
Z-L-tryptophan
0.000012 - 0.00023
Zincov
0.0012
[(2S)-2-sulfanyl-3-phenylpropanoyl]Gly-(5-Ph)Pro
pH 8.0, 37°C
0.000042
[(2S)-2-sulfanyl-3-phenylpropanoyl]Phe-Tyr
pH 8.0, 37°C
0.000048
[(2S,R)-2-sulfanylheptanoyl]Phe-Ala
pH 8.0, 37°C
additional information
additional information
-