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3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

Specific Activity

Specific Activity on EC 3.4.24.27 - thermolysin

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.34
-
mutant F114H, substrate casein, 25°C, pH 7.5
0.55
-
mutant N227A, substrate casein, 25°C, pH 7.5
0.62
-
mutant D150W, substrate casein, 25°C, pH 7.5
0.68
-
mutant I168A, substrate casein, 25°C, pH 7.5
0.84
-
mutant D150H, substrate casein, 25°C, pH 7.5
0.93
-
mutant D150E, substrate casein, 25°C, pH 7.5
1.08
-
wild-type, substrate casein, 25°C, pH 7.5
1.14
-
mutant F114A, substrate casein, 25°C, pH 7.5
1.18
-
mutant N227H, substrate casein, 25°C, pH 7.5
10.2
-
purified mutant enzyme S53D/L155A, using casein as substrate, at pH 7.5 and 25°C
10.5
-
purified mutant enzyme D150E, using casein as substrate, at pH 7.5 and 25°C
11.3
-
purified mutant enzyme S53D, using casein as substrate, at pH 7.5 and 25°C
11.9
-
purified mutant enzyme S53D/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C
11000
-
recombinant wild-type enzyme, substrate casein, pH 7.5, 25°C
12000
1468
purified native enzyme, pH 8.0, 30°C, substrate bovine serum albumin
2000
-
recombinant mutant G117D, substrate casein, pH 7.5, 25°C
2899
purified native enzyme, pH 8.0, 30°C, substrate gelatin
2900
-
recombinant mutant G117R, substrate casein, pH 7.5, 25°C
3.5
-
purified mutant enzyme L144S/D150E, using casein as substrate, at pH 7.5 and 25°C
3.6
-
purified mutant enzyme L144S, using casein as substrate, at pH 7.5 and 25°C
3077
purified native enzyme, pH 8.0, 30°C, substrate casein
3200
-
recombinant mutant G117K, substrate casein, pH 7.5, 25°C
373
purified native enzyme, pH 8.0, 30°C, substrate feather
489
purified native enzyme, pH 8.0, 30°C, substrate elastin
5300
purified recombinant mutant N116A, pH 7.5, 25°C, substrate N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
5400
purified recombinant mutant N116T, pH 7.5, 25°C, substrate N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
6.7
-
purified mutant enzyme L155A, using casein as substrate, at pH 7.5 and 25°C
6800
purified recombinant mutant N116Q, pH 7.5, 25°C, substrate N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
697
purified native enzyme, pH 8.0, 30°C, substrate hemoglobin
7.5
-
purified mutant enzyme D150E/I168A, using casein as substrate, at pH 7.5 and 25°C
7.9
-
purified mutant enzyme S53D/L155A/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C
7900
purified recombinant mutant N116D, pH 7.5, 25°C, substrate N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
8.1
-
purified mutant enzyme L155A/G8C/N60C/S65P, using casein as substrate, at pH 7.5 and 25°C
855
purified native enzyme, pH 8.0, 30°C, substrate collagen
9.6
-
purified mutant enzyme I168A, using casein as substrate, at pH 7.5 and 25°C
9340
-
pH and temperature not specified in the publication
9500
-
pH 7.5, 25°C
additional information