3.1.30.2: Serratia marcescens nuclease
This is an abbreviated version!
For detailed information about Serratia marcescens nuclease, go to the full flat file.
Word Map on EC 3.1.30.2
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3.1.30.2
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strand
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single-stranded
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nick
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chromatin
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exonuclease
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phage
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nucleases
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rnase
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bacteriophage
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endonucleolytic
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mismatch
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palindromic
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structure-specific
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internucleosomal
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homing
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glycosylase
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methylase
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phosphodiester
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fork
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restriction-modification
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interspaced
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supercoiled
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crispr
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heteroduplexes
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abasic
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deoxyribonuclease
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retrotransposons
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xeroderma
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aif
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caspase-independent
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dsdna
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pigmentosum
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ape1
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endoribonuclease
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holliday
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end-joining
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nucleolytic
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colicins
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ecorv
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retrotransposition
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interstrand
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okazaki
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mre11
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superhelical
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pvuii
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haeiii
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overhang
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cap-dependent
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sgrnas
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resolvase
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molecular biology
- 3.1.30.2
- strand
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single-stranded
- nick
- chromatin
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exonuclease
- phage
- nucleases
- rnase
- bacteriophage
-
endonucleolytic
- mismatch
-
palindromic
-
structure-specific
-
internucleosomal
-
homing
- glycosylase
-
methylase
-
phosphodiester
- fork
-
restriction-modification
-
interspaced
-
supercoiled
-
crispr
- heteroduplexes
-
abasic
- deoxyribonuclease
-
retrotransposons
- xeroderma
- aif
-
caspase-independent
- dsdna
- pigmentosum
- ape1
-
endoribonuclease
-
holliday
-
end-joining
-
nucleolytic
- colicins
- ecorv
-
retrotransposition
-
interstrand
-
okazaki
- mre11
-
superhelical
- pvuii
- haeiii
-
overhang
-
cap-dependent
-
sgrnas
- resolvase
- molecular biology
Reaction
endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =
Synonyms
barley nuclease, BFN1, BMN, CAD, caspase-activated DNase, DFF, DFF40/CAD endonuclease, DNA fragmentation factor, EC 3.1.4.9, ENDO1, ENDO2, ENDO4, ENDO5, endonuclease, endonuclease (Serratia marcescens), endonuclease 1, endonuclease 2, endonuclease 4, endonuclease 5, Kamchatka crab duplex-specific nuclease, More, mung bean nuclease, NUC49, nucA, NucANLS, nuclease A, nuclease I, nuclease, nucleate endo-, nucleate endonuclease, Par_DSN, plant nuclease I, plant S1-like nuclease, plant type I nuclease, rNUC49, S1-like nuclease, Serratia marcescens endonuclease, Serratia marcescens nuclease, Sm2, Sma, Sma nuc, Sma nuc endonuclease, SMnase
ECTree
3.1.30.2 Serratia marcescens nucleaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.1.30.2 - Serratia marcescens nuclease
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5'-CCTGGCAGTT + H2O
?
-
synthetic labeled deoxydecanucleotide, cleavage of 5'-Ap*G bond and with lower activity of Gp*T, Cp*A, and Gp*G bonds
-
-
?
B-Z-hybrid form DNA + H2O
?
-
hybrid B-Z form of herring testis DNA
-
-
?
lambda DNA + H2O
?
-
-
size of linear DNA fragments decreases with prolonged incubation time
-
?
pUC18 DNA + H2O
?
-
covalently closed circular plasmid DNA
substrate converted into relaxed circular form and than to the linear form
-
?
pUC19 DNA + H2O
?
-
relaxation of the supercoiled DNA and cutting of the open circular DNA to a linear form
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
Azotobacter agilis
-
-
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
heat denatured
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
native double-stranded
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
partially degraded DNA
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
hydrolysis rate about 3 times more slowly than compared to RNA
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
single-stranded DNA
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
heat denatured
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
native double-stranded
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
cleaves supercoiled DNA only at specific sites
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
single-stranded DNA
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
cleaves supercoiled DNA only at specific sites
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
-
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
DNA is slightly preferred as a substrate than RNA
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
cleaves supercoiled DNA only at specific sites
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
cleaves supercoiled DNA only at specific sites
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
native double-stranded
-
-
?
DNA + H2O
5'-phosphonucleotides + 5'-phosphomononucleotides
-
single-stranded DNA
-
-
?
DNA + H2O
?
-
tritium labeled DNA from Escherichia coli, fragmented by limited sonication
random DNA cleavage with respect to all possible phosphodiester bonds
-
?
DNA + H2O
?
dsDNA to ssDNA preference ratio for Par_DSN-t4 is equal to 10, corresponding ratio for intact Par_DSN is equal to 1000
-
-
?
DNA + H2O
?
protein and DNA are held together by a mix of salt-bridges, water-mediated and direct hydrogen bond interactions between the protein and DNA backbone, almost no DNA bases are directly involved in the contacts
-
-
?
DNA + H2O
?
in absence of Mg2+ the enzyme shows a preference for DNA as compared to RNA
-
-
?
DNA + H2O
?
Serratia marcescens nuclease is highly effective on sputum
-
-
?
dsDNA + H2O
?
-
primarily responsible for internucleosomal DNA cleavage during the terminal stages of apoptosis, preference for cleaving the internucleosomal linker regions in chromatin
fragments possessing ends with 5-phosphate and 3-hydroxyl groups, exclusively double strand breaks (primarily blunt ends)
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?
dsDNA + H2O
?
-
-
DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends
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?
dsDNA + H2O
?
-
physiological function currently unknown
DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
Azotobacter agilis
-
polyuridylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
Azotobacter agilis
-
polycytidylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
Azotobacter agilis
-
e.g.: polyadenylic acid
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-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
Azotobacter agilis
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not polyguanylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
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polyguanylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
rRNA
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
-
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
-
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
-
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
polyuridylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
polycytidylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
e.g.: polyadenylic acid
-
-
?
RNA + H2O
5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
highest activity against poly(U)
-
-
?
RNA + H2O
?
in absence of Mg2+ the enzyme shows a preference for DNA as compared to RNA
-
-
?
additional information
?
-
isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme
-
-
?
additional information
?
-
isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme
-
-
?
additional information
?
-
isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme
-
-
?
additional information
?
-
isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme
-
-
?
additional information
?
-
isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity
-
-
?
additional information
?
-
isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity
-
-
?
additional information
?
-
isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity
-
-
?
additional information
?
-
isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity
-
-
?
additional information
?
-
no activity by isozyme ENDO5 on ssDNA or dsDNA
-
-
?
additional information
?
-
no activity by isozyme ENDO5 on ssDNA or dsDNA
-
-
?
additional information
?
-
no activity by isozyme ENDO5 on ssDNA or dsDNA
-
-
?
additional information
?
-
no activity by isozyme ENDO5 on ssDNA or dsDNA
-
-
?
additional information
?
-
the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA
-
-
?
additional information
?
-
the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA
-
-
?
additional information
?
-
the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA
-
-
?
additional information
?
-
the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA
-
-
?
additional information
?
-
Azotobacter agilis
-
hydrolysis of shorter chains much slower than of longer chains
-
-
?
additional information
?
-
-
enzyme also shows 3'-nucleotidase activity
-
-
?
additional information
?
-
-
single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are not substrates for the enzyme
-
-
?
additional information
?
-
-
inactive towards 3'-phosphoester linkage of nucleoside cyclic 2',3'- and 3',5'-monophosphates
-
-
?
additional information
?
-
-
3'-nucleotidase activity is greater for purine than for pyrimidine ribonucleotides
-
-
?
additional information
?
-
-
little activity towards ribonucleoside 2'- and 5'-monophosphates and deoxyribonucleoside 3'- and 5'-monophosphates
-
-
?
additional information
?
-
-
poly(dC)poly(dG) is not substrate for the enzyme
-
-
?
additional information
?
-
-
recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand
-
-
?
additional information
?
-
-
linear flap structures with tails of more than 20 nucleotides and shorter duplex regions are not hydrolyzed
-
-
?
additional information
?
-
-
the subunits of the dimer function independently as monomers, molecular dynamic simulations involving Mg2+, modelling of complex building with DNA
-
-
?
additional information
?
-
-
the enzyme potently degrades both DNA and RNA and is a nuclease with broad specificity, but it shows some sensitivity to the secondary structure of the substrate
-
-
?
additional information
?
-
-
substrate is highly polymerized herring testis DNA of the random nucleotide sequence. Hydrolysis of the B-form and hybrid B-Z form DNA by the enzyme. The hybrid B-Z form is formed upon addition of MgSO4 and Co(NH3)6Cl3
-
-
?
additional information
?
-
-
substrate specificity in descending order is ssDNA, dsDNA, RNA
-
-
?
additional information
?
-
-
substrate specificity, no activity with dinucleoside monophosphates, overview
-
-
?
additional information
?
-
-
mung bean nuclease is an endonuclease specific to single-stranded DNA or RNA
-
-
?
