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Literature summary for 3.1.30.2 extracted from

  • Hanus, J.; Kalinowska-Herok, M.; Widlak, P.
    The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme (2008), Apoptosis, 13, 377-382.
    View publication on PubMed
Search for more literature for 3.1.30.2

Activating Compound

Activating Compound Comment Organism Structure
caspase-3 cleavage activates inactive enzyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
His-tag fusion protein expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Double-stranded RNA
-
 Homo sapiens
RNA-DNA heteroduplexes
-
 Homo sapiens
single-stranded DNA
-
 Homo sapiens
Single-stranded RNA
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Homo sapiens primarily responsible for internucleosomal DNA cleavage during the terminal stages of apoptosis, preference for cleaving the internucleosomal linker regions in chromatin ? fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, exclusively double strand breaks (primarily blunt ends) ?
additional information Homo sapiens single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are not substrates for the enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O primarily responsible for internucleosomal DNA cleavage during the terminal stages of apoptosis, preference for cleaving the internucleosomal linker regions in chromatin Homo sapiens ? fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, exclusively double strand breaks (primarily blunt ends) ?
additional information single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are not substrates for the enzyme Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, crystal structure analysis Homo sapiens

Synonyms

Synonyms Comment Organism
CAD
-
 Homo sapiens
caspase-activated DNase
-
 Homo sapiens
DFF
-
 Homo sapiens
DFF40/CAD endonuclease
-
 Homo sapiens
DNA fragmentation factor
-
 Homo sapiens