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Literature summary for 3.1.30.2 extracted from

  • Yu, Z.; Cao, K.; Tischler, T.; Stolle, C.A.; Santani, A.B.
    Mung bean nuclease treatment increases capture specificity of microdroplet-PCR based targeted DNA enrichment (2014), PLoS ONE, 9, e103491.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
molecular biology the enzyme is useful in DNA enrichment by PCR based applications for which removal of template DNA is beneficial before end repair, method overview. Mung bean nuclease treatment abolished the high molecular DNA smear in microdroplet-PCR captured DNA after end repair Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vigna radiata mung bean nuclease is an endonuclease specific to single-stranded DNA or RNA ?
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 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mung bean nuclease is an endonuclease specific to single-stranded DNA or RNA Vigna radiata ?
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 ?
additional information human DNA samples are used as substrates Vigna radiata ?
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Synonyms

Synonyms Comment Organism
mung bean nuclease
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 Vigna radiata

General Information

General Information Comment Organism
additional information treatment of enriched DNA with the mung bean nuclease, an endonuclease specific to single-stranded DNA or RNA, can dramatically reduce genomic DNA carry over of single-stranded template genomic DNA from microdroplet-PCR and increase on-target efficiency of the resultant library. Nuclease treatment of enrichment products shall be incorporated in the workflow of targeted sequencing using microdroplet-PCR for enrichment Vigna radiata