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Literature summary for 3.1.30.2 extracted from

  • Imagawa, H.; Toryu, H.; Ozawa, T.; Takino, Y.
    Purification and characterization of nucleases from tea leaves (1982), Agric. Biol. Chem., 46, 1261-1269.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Camellia sinensis
Cu2+
-
Camellia sinensis
EDTA
-
Camellia sinensis
Fe3+
-
Camellia sinensis
Mg2+
-
Camellia sinensis
Mn2+ inhibits hydrolysis of RNA, denatured DNA and 3'-AMP, not: native DNA Camellia sinensis
p-chloromercuribenzoate
-
Camellia sinensis
Zn2+
-
Camellia sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ inhibition Camellia sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
form B, gel filtration Camellia sinensis
35000
-
form A, gel filtration Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
tea, 2 forms: A, B
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: A, B Camellia sinensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Camellia sinensis

Storage Stability

Storage Stability Organism
4°C, pH 5.5, 0.04 M sodium acetate buffer, 4 months Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-nucleotides + H2O e.g. 3'-AMP Camellia sinensis ?
-
?
DNA + H2O heat denatured Camellia sinensis 5'-phosphonucleotides + 5'-phosphomononucleotides
-
?
DNA + H2O native double-stranded Camellia sinensis 5'-phosphonucleotides + 5'-phosphomononucleotides
-
?
additional information enzyme also shows 3'-nucleotidase activity Camellia sinensis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70 nuclease B: RNA, denatured DNA, 3'-AMP Camellia sinensis
70
-
nuclease A, RNA, denatured DNA, 3'-AMP Camellia sinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: 50% nuclease B, 10% nuclease A of activity maximum, 80°C: 30% nuclease B, 60% nuclease A of activity maximum Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
below Camellia sinensis
80
-
15 min, 80% loss of activity Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
native DNA Camellia sinensis
6
-
RNA, denatured DNA Camellia sinensis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
Camellia sinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 4°C Camellia sinensis