BRENDA - Enzyme Database show
show all sequences of 3.1.30.2

Is crab duplex-specific nuclease a member of the Serratia family of non-specific nucleases?

Anisimova, V.E.; Shcheglov, A.S.; Bogdanova, E.A.; Rebrikov, D.V.; Nekrasov, A.N.; Barsova, E.V.; Shagin, D.A.; Lukyanov, S.A.; Gene 418, 41-48 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
Par_DSN-t1: residues 192-379 of Par_DSN precursor (GenBank ID AAN86143) linked with a bacterial signal peptide and 6×His tag (sequence corresponds to the NUC domain). Par_DSN-t2: residues 149-406, corresponds to the NUC domain identified by multiple sequence alignment, is sensitive to proteinase K and is completely cleaved by this protease. Par_DSN-t3: residues 162-406, additionaly all unpaired Cys residues are deleted, Par_DSN-t3 is soluble, sensitive to proteinase K. Par_DSN-t4: residues 114-406, Par_DSN-t4 is degraded by proteinase K
Paralithodes camtschaticus
Engineering
Amino acid exchange
Commentary
Organism
G133V
yield after proteinase K treatment 0%
Paralithodes camtschaticus
G139V
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H168A
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H171A
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H237A
yield after proteinase K treatment 20%
Paralithodes camtschaticus
K235A
yield after proteinase K treatment 19%
Paralithodes camtschaticus
K235R
yield after proteinase K treatment 21%
Paralithodes camtschaticus
R121/122A
yield after proteinase K treatment 4%
Paralithodes camtschaticus
R121A
for specific activity measurement
Paralithodes camtschaticus
R121K
yield after proteinase K treatment 19%
Paralithodes camtschaticus
R122A
for specific activity measurement
Paralithodes camtschaticus
R122K
yield after proteinase K treatment 20%
Paralithodes camtschaticus
R184A
yield after proteinase K treatment 22%
Paralithodes camtschaticus
R184K
yield after proteinase K treatment 18%
Paralithodes camtschaticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DNA + H2O
Paralithodes camtschaticus
dsDNA to ssDNA preference ratio for Par_DSN-t4 is equal to 10, corresponding ratio for intact Par_DSN is equal to 1000
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paralithodes camtschaticus
Q8I9M9
-
-
Purification (Commentary)
Commentary
Organism
Par_DSN-t1 is insoluble and inactive after puriciation and renaturation
Paralithodes camtschaticus
Renatured (Commentary)
Commentary
Organism
Par_DSN-t1 is insoluble and inactive after purification and renaturation. Par_DSN-t2, Par_DSN-t3, Par_DSN-t4 could be renaturated.
Paralithodes camtschaticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
G133V mutant, dsDNA, not detectable (inactive protein); G139V mutant, dsDNA, not detectable (inactive protein); H168A mutant, dsDNA, not detectable (inactive protein); H171A mutant, dsDNA, not detectable (inactive protein); H237A mutant, dsDNA, not detectable; K235A mutant, dsDNA, 110 Kunitz units; K235R mutant, dsDNA, 5980 Kunitz units; Par_DSN-t1 lacks DNAse activity; Par_DSN-t2 lacks DNase activity; Par_DSN-t3 lacks DNAse activity; Par_DSN-t4 has low specific activity (6 Kunitz-units compared to 6070 Kunitz units for intact Par_DSN), dsDNA; R121/122A mutant, dsDNA, not detectable (inactive protein); R121A mutant, dsDNA, 5960 Kunitz units; R121K mutant, dsDNA, 5990 Kunitz units; R122A mutant, dsDNA, 6030 Kunitz units; R122K mutant, dsDNA, 6050 Kunitz units; R184A mutant, dsDNA, 1810 Kunitz units; R184K mutant, dsDNA, 6100 Kunitz units; wild-type, dsDNA, 6070 Kunitz units
Paralithodes camtschaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DNA + H2O
dsDNA to ssDNA preference ratio for Par_DSN-t4 is equal to 10, corresponding ratio for intact Par_DSN is equal to 1000
698091
Paralithodes camtschaticus
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
Par_DSN-t4
Paralithodes camtschaticus
60
-
Par_DSN
Paralithodes camtschaticus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
activity of Par_DSN-t4 completely disappears after incubation for 20 min, full-length Par_DSN retains 25% activity
Paralithodes camtschaticus
Cloned(Commentary) (protein specific)
Commentary
Organism
Par_DSN-t1: residues 192-379 of Par_DSN precursor (GenBank ID AAN86143) linked with a bacterial signal peptide and 6×His tag (sequence corresponds to the NUC domain). Par_DSN-t2: residues 149-406, corresponds to the NUC domain identified by multiple sequence alignment, is sensitive to proteinase K and is completely cleaved by this protease. Par_DSN-t3: residues 162-406, additionaly all unpaired Cys residues are deleted, Par_DSN-t3 is soluble, sensitive to proteinase K. Par_DSN-t4: residues 114-406, Par_DSN-t4 is degraded by proteinase K
Paralithodes camtschaticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G133V
yield after proteinase K treatment 0%
Paralithodes camtschaticus
G139V
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H168A
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H171A
yield after proteinase K treatment 0%
Paralithodes camtschaticus
H237A
yield after proteinase K treatment 20%
Paralithodes camtschaticus
K235A
yield after proteinase K treatment 19%
Paralithodes camtschaticus
K235R
yield after proteinase K treatment 21%
Paralithodes camtschaticus
R121/122A
yield after proteinase K treatment 4%
Paralithodes camtschaticus
R121A
for specific activity measurement
Paralithodes camtschaticus
R121K
yield after proteinase K treatment 19%
Paralithodes camtschaticus
R122A
for specific activity measurement
Paralithodes camtschaticus
R122K
yield after proteinase K treatment 20%
Paralithodes camtschaticus
R184A
yield after proteinase K treatment 22%
Paralithodes camtschaticus
R184K
yield after proteinase K treatment 18%
Paralithodes camtschaticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DNA + H2O
Paralithodes camtschaticus
dsDNA to ssDNA preference ratio for Par_DSN-t4 is equal to 10, corresponding ratio for intact Par_DSN is equal to 1000
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Par_DSN-t1 is insoluble and inactive after puriciation and renaturation
Paralithodes camtschaticus
Renatured (Commentary) (protein specific)
Commentary
Organism
Par_DSN-t1 is insoluble and inactive after purification and renaturation. Par_DSN-t2, Par_DSN-t3, Par_DSN-t4 could be renaturated.
Paralithodes camtschaticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
G133V mutant, dsDNA, not detectable (inactive protein); G139V mutant, dsDNA, not detectable (inactive protein); H168A mutant, dsDNA, not detectable (inactive protein); H171A mutant, dsDNA, not detectable (inactive protein); H237A mutant, dsDNA, not detectable; K235A mutant, dsDNA, 110 Kunitz units; K235R mutant, dsDNA, 5980 Kunitz units; Par_DSN-t1 lacks DNAse activity; Par_DSN-t2 lacks DNase activity; Par_DSN-t3 lacks DNAse activity; Par_DSN-t4 has low specific activity (6 Kunitz-units compared to 6070 Kunitz units for intact Par_DSN), dsDNA; R121/122A mutant, dsDNA, not detectable (inactive protein); R121A mutant, dsDNA, 5960 Kunitz units; R121K mutant, dsDNA, 5990 Kunitz units; R122A mutant, dsDNA, 6030 Kunitz units; R122K mutant, dsDNA, 6050 Kunitz units; R184A mutant, dsDNA, 1810 Kunitz units; R184K mutant, dsDNA, 6100 Kunitz units; wild-type, dsDNA, 6070 Kunitz units
Paralithodes camtschaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DNA + H2O
dsDNA to ssDNA preference ratio for Par_DSN-t4 is equal to 10, corresponding ratio for intact Par_DSN is equal to 1000
698091
Paralithodes camtschaticus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
Par_DSN-t4
Paralithodes camtschaticus
60
-
Par_DSN
Paralithodes camtschaticus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
activity of Par_DSN-t4 completely disappears after incubation for 20 min, full-length Par_DSN retains 25% activity
Paralithodes camtschaticus
Other publictions for EC 3.1.30.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730531
Filimonova
-
Some features of hydrolysis of ...
Serratia marcescens
OnLine J. Biol. Sci.
14
179-185
2014
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
730764
Yu
Mung bean nuclease treatment i ...
Vigna radiata
PLoS ONE
9
e103491
2014
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730587
Lesniewicz
The plant S1-like nuclease fam ...
Arabidopsis thaliana
Plant Cell Physiol.
54
1064-1078
2013
-
-
1
-
-
-
1
-
-
4
-
4
-
4
-
-
-
-
-
8
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
12
-
4
-
-
-
-
-
14
-
-
4
-
-
-
-
-
4
-
-
-
-
2
8
-
-
-
730959
Romanova
The effects of addition of mon ...
Serratia marcescens
ScientificWorldJournal
2012
454176
2012
-
-
-
-
-
-
5
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
5
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
695826
Li
A fluorescent, genetically eng ...
Serratia marcescens
Appl. Microbiol. Biotechnol.
82
749-756
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699775
Chen
Advantage of being a dimer for ...
Serratia marcescens
J. Phys. Chem. B
113
511-521
2009
-
-
1
-
-
-
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701699
Caballero
Evaluation of the Serratia mar ...
Serratia marcescens
Anim. Biotechnol.
20
177-185
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
677620
Hanus
The major apoptotic endonuclea ...
Homo sapiens
Apoptosis
13
377-382
2008
1
-
1
-
-
-
4
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698091
Anisimova
Is crab duplex-specific nuclea ...
Paralithodes camtschaticus
Gene
418
41-48
2008
-
-
1
-
14
-
-
-
-
-
-
1
-
4
-
-
1
-
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
678425
Laquel-Robert
Identification and biochemical ...
Podospora anserina
Biochim. Biophys. Acta
1770
527-542
2007
1
-
1
-
-
-
4
-
3
1
1
4
-
5
-
-
1
-
-
-
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
3
1
1
4
-
-
-
1
-
-
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666881
Chen
Solvent participation in Serra ...
Serratia marcescens
Proteins
62
982-995
2006
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680576
Mandal
Purification and characterizat ...
Mycolicibacterium smegmatis
J. Biochem. Mol. Biol.
39
140-144
2006
-
-
-
-
-
1
3
-
-
2
1
2
-
4
-
-
1
-
-
-
1
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
2
1
2
-
-
-
1
-
-
1
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665188
Pires de Castro
-
Mechanism of DNA cleavage cata ...
Vigna radiata
Inorg. Chim. Acta
357
2579-2592
2004
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666651
Yupsanis
Purification, properties and s ...
Thinopyrum elongatum
Plant Physiol. Biochem.
42
795-802
2004
-
-
-
-
-
-
10
-
-
2
2
4
-
5
-
-
1
-
-
4
1
-
12
1
1
-
2
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
-
2
2
4
-
-
-
1
-
4
1
-
12
1
1
-
2
-
1
-
-
1
-
-
-
-
-
-
654944
Filimonova
Action of hexaamminecobalt on ...
Serratia marcescens
BioMetals
16
447-453
2003
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655293
Berkmen
Multi-copy repression of Serra ...
Serratia marcescens
Curr. Microbiol.
44
44-48
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657343
Shlyapnikov
-
A comparative structure-functi ...
Serratia marcescens
Russ. J. Bioorg. Chem.
28
20-27
2002
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654960
Koziolkiewicz
Stereochemistry of cleavage of ...
Serratia marcescens
Bioorg. Med. Chem.
9
2403-2409
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657122
Marchetti
Isolation and characterization ...
Hordeum vulgare
Planta
213
199-206
2001
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
2
-
-
12
-
1
1
3
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
2
-
-
12
-
1
1
3
-
1
1
2
-
-
-
-
-
-
-
135016
Shlyapnikov
Atomic structure of the Serrat ...
Serratia marcescens
Acta Crystallogr. Sect. D
56
567-572
2000
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135022
Lunin
-
Extracellular endonuclease of ...
Serratia marcescens
Mol. Biol.
33
180-187
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135015
Franke
Genetic engineering, productio ...
Serratia marcescens
FEBS Lett.
425
517-522
1998
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135018
Kobayashi
Purification and characterizat ...
Lentinula edodes
Biosci. Biotechnol. Biochem.
59
1169-1171
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
1
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
1
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135017
Friedhoff
A procedure for renaturation a ...
Serratia marcescens
Protein Expr. Purif.
5
37-43
1994
-
-
1
-
-
-
-
-
2
-
-
-
-
3
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135020
Filimonova
Kinetic studies of the Serrati ...
Serratia marcescens
Biochem. Mol. Biol. Int.
33
1229-1236
1994
-
-
-
-
-
-
4
-
1
1
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135021
Pedersen
Characterization of Serratia m ...
Serratia marcescens
Biochim. Biophys. Acta
1202
13-21
1993
-
-
1
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135014
Grafi
-
Characterization of S1/mung-be ...
Nicotiana tabacum, Petunia x hybrida, Solanum lycopersicum, Triticum monococcum
Plant Sci.
74
107-114
1991
-
-
-
-
-
-
8
-
4
-
-
-
-
4
-
-
-
-
-
8
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
4
-
-
-
-
-
-
-
-
8
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135019
Bannikova
Two isoforms of Serratia marce ...
Serratia marcescens
Biochem. Int.
24
813-822
1991
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
95075
Brown
Biochemical properties and hor ...
Hordeum vulgare
Eur. J. Biochem.
168
357-364
1987
-
-
-
-
-
-
3
-
-
3
2
-
-
4
-
1
-
-
-
1
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
2
-
-
-
1
-
-
1
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135010
Sawicka
-
Membrane-bound nucleolytic act ...
Zea mays
Phytochemistry
26
59-63
1987
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135012
Varlamov
Ligand-exchange chromatography ...
Serratia marcescens
J. Chromatogr.
364
215-223
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
3
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
95082
Imagawa
Purification and characterizat ...
Camellia sinensis
Agric. Biol. Chem.
46
1261-1269
1982
-
-
-
-
-
-
8
-
-
1
2
-
-
1
-
-
1
-
-
-
1
1
4
-
2
1
2
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
2
-
-
-
-
1
-
-
1
1
4
-
2
1
2
-
2
1
1
-
-
-
-
-
-
-
134499
Pietrzak
Purification and properties of ...
Hordeum vulgare
Biochim. Biophys. Acta
614
102-112
1980
-
-
-
-
-
-
7
-
-
1
1
-
-
3
-
-
1
-
-
1
1
-
2
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
1
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
135008
Sasakuma
-
Partial purification and prope ...
Hordeum vulgare
Phytochemistry
18
1873-1874
1979
-
-
-
-
-
-
3
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135011
Oleson
An extracellular nuclease from ...
Nicotiana tabacum
Biochim. Biophys. Acta
366
89-100
1974
-
-
-
-
-
-
1
-
1
1
1
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
1
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
135005
Stevens
-
Studies on a nuclease from Azo ...
Azotobacter agilis
J. Biol. Chem.
235
3016-3022
1960
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135006
Stevens
-
Studies on a nuclease from Azo ...
Azotobacter agilis
J. Biol. Chem.
235
3023-3027
1960
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-