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Literature summary for 3.1.30.2 extracted from
- Studies on a nuclease from Azotobacter agilis. I. Isolation and mode of action (1960), J. Biol. Chem., 235, 3016-3022.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | secondary interaction in long RNA chains inhibit the enzyme | Azotobacter agilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter agilis | - |
similar enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| similar enzyme | Azotobacter agilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Azotobacter agilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Azotobacter agilis | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? |  |
| additional information | hydrolysis of shorter chains much slower than of longer chains | Azotobacter agilis | ? | - |
? | |
| RNA + H2O | polyuridylic acid | Azotobacter agilis | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
| RNA + H2O | polycytidylic acid | Azotobacter agilis | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
| RNA + H2O | e.g.: polyadenylic acid | Azotobacter agilis | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
| RNA + H2O | not polyguanylic acid | Azotobacter agilis | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | similar enzymes: silkworm nuclease, potato nuclease, Azotobacter nuclease | Azotobacter agilis |
| More | mung bean nuclease has specificity for single-stranded substrates, see E.C. 3.1.30.1 | Azotobacter agilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Azotobacter agilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 8.5 | sharp decrease in activity below and above | Azotobacter agilis |
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Release 2023.1 (January 2023)
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