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Literature summary for 3.1.30.2 extracted from

  • Li, Q.; Wu, Y.J.
    A fluorescent, genetically engineered microorganism that degrades organophosphates and commits suicide when required (2009), Appl. Microbiol. Biotechnol., 82, 749-756.
    View publication on PubMed
Search for more literature for 3.1.30.2

Cloned(Commentary)

Cloned (Comment) Organism
generation of plasmids pSS and pDS (for induction of suicide mechanism) consisting of kanamycin resistance, T7-promotor, Serratia marcescens nuclease (1 copy in pSS, 2 copies in pDS) minus its lead-peptide-coding nucleotide sequence. cotransformation with plasmid pL-EGFP-OPH (containing organophosphorus hydrolase and enhanced green fluorescent protein for degradation of organophosphates) of Escherichia coli strain BL21-AI(TM) to generate BL21AI-GOS (pDS) Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Serratia marcescens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cells transformed with plasmid pDS (carrying 2 copies of Serratia marcescens nuclease) are more effective in stopping cell division than those transformed with plasmid pSS (carrying 1 copy of Serratia marcescens nuclease), OD600 analysis, colony test on plates. Cells cotransformed grow more slowly before induction of Serratia marcescens nuclease, OD600 analysis, florescence, colony test on plates Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Serratia marcescens ?
-
 ?

Synonyms

Synonyms Comment Organism
Serratia marcescens nuclease
-
 Serratia marcescens