3.1.30.2: Serratia marcescens nuclease

This is an abbreviated version!
For detailed information about Serratia marcescens nuclease, go to the full flat file.

Word Map on EC 3.1.30.2

3.1.30.2

strand

single-stranded

nick

chromatin

exonuclease

phage

nucleases

rnase

bacteriophage

endonucleolytic

mismatch

palindromic

structure-specific

internucleosomal

homing

glycosylase

methylase

phosphodiester

fork

restriction-modification

interspaced

supercoiled

crispr

heteroduplexes

abasic

deoxyribonuclease

retrotransposons

xeroderma

aif

caspase-independent

dsdna

pigmentosum

ape1

endoribonuclease

holliday

end-joining

nucleolytic

colicins

ecorv

retrotransposition

interstrand

okazaki

mre11

superhelical

pvuii

haeiii

overhang

cap-dependent

sgrnas

resolvase

molecular biology

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

barley nuclease, BFN1, BMN, CAD, caspase-activated DNase, DFF, DFF40/CAD endonuclease, DNA fragmentation factor, EC 3.1.4.9, ENDO1, ENDO2, ENDO4, ENDO5, endonuclease, endonuclease (Serratia marcescens), endonuclease 1, endonuclease 2, endonuclease 4, endonuclease 5, Kamchatka crab duplex-specific nuclease, More, mung bean nuclease, NUC49, nucA, NucANLS, nuclease A, nuclease I, nuclease, nucleate endo-, nucleate endonuclease, Par_DSN, plant nuclease I, plant S1-like nuclease, plant type I nuclease, rNUC49, S1-like nuclease, Serratia marcescens endonuclease, Serratia marcescens nuclease, Sm2, Sma, Sma nuc, Sma nuc endonuclease, SMnase

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters

3.1.30.2 Serratia marcescens nuclease

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Results	in table
3	Activating Compound
39	AA Sequence
1	Application
1	CAS Registry Number
14	Cloned(Commentary)
3	Crystallization (Commentary)
2473	Disease/ Diagnostics
1	Expression
14	General Information
1	General Stability
68	Inhibitors
6	Ki Value [mM]
8	KM Value [mM]
11	Localization
35	Metals/Ions
15	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
49	Organism
10	PDB ID
20	pH Optimum
8	pH Range
3	pH Stability
1	pI Value
1	Posttranslational Modification
15	Protein Variants
21	Purification (Commentary)
6	Reaction
4	Reaction Type
45	Reference
2	Renatured (Commentary)
29	Source Tissue
28	Specific Activity [micromol/min/mg]
2	Storage Stability
116	Substrates and Products (Substrate)
9	Subunits
62	Synonyms
9	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 3.1.30.2 - Serratia marcescens nuclease

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2 forms: A, B

2 isoforms

135012, 135019, 135020

affinity chromatography, 63fold purification

Hordeum vulgare

657122

ion exchange chromatography on DEAE-cellulose DE-32 and DEAE-cellulose DE-52

Serratia marcescens

654944

native enzyme 265fold to homogeneity from seedlings by ammonium sulfate fractionation, anion exchange chromatography and heparin affinity chromatography

nuclease Le3

one-step protocol for chromatographic purification. The purification procedure provides substantial yields of homogenously pure and highly active enzyme

partial

Par_DSN-t1 is insoluble and inactive after puriciation and renaturation

Paralithodes camtschaticus

Q8I9M9

698091

purified to near homogeneity

Mycolicibacterium smegmatis

680576

recombinant protein using His-tag

2 entries

similar enzyme

Azotobacter agilis

135005

Sm1 and Sm2

Serratia marcescens

135020