3.1.1.22: hydroxybutyrate-dimer hydrolase

This is an abbreviated version!
For detailed information about hydroxybutyrate-dimer hydrolase, go to the full flat file.

Word Map on EC 3.1.1.22

Reaction

(R)-3-((R)-3-hydroxybutanoyloxy)butanoate
+
H2O
= 2 (R)-3-hydroxybutanoate

Synonyms

More, D-(-)-3-hydroxybutyrate-dimer hydrolase, 3HB-oligomer hydrolase, hydrolase, hydroxybutyrate dimer, D-(-)-3-hydroxybutyrate oligomer hydrolase, 3HB oligomer hydrolase, D(-)-3-hydroxybutyrate oligomer hydrolase, PhaZ1, PhaZ, 3-hydroxybutyrate-oligomer hydrolase, 3-HB-oligomer hydrolase, 3HBOH, 3-hydroxybutyrate oligomer hydrolase, PhaZc, PhaZ2, H16 A2862, poly[D-(-)-3-hydroxybutyrate] depolymerase, intracellular PHB depolymerase, phaZre

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.22 hydroxybutyrate-dimer hydrolase

Cloned

Cloned on EC 3.1.1.22 - hydroxybutyrate-dimer hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, functional expression in Escherichia coli as His-tagged enzyme, in vitro transcription and translation
expressed in Escherichia coli JM109 cells
expression in Escherichia coli
gene i3HBOH, DNA sequence determination and analysis, expression in Escherichia coli
gene phaZ1, pha sequences comparisons, subcloning in Escherichia coli strains DH5alpha and S17-1
gene phaZ2, pha sequences comparisons, subcloning in strains DH5alpha and S17-1