Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paucimonas lemoignei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 1,4-dimercapto-2,3-butanediol; not inhibited by (p-tert-octylphenoxy)polyethoxyethanol | Paucimonas lemoignei | |
Phenylmethylsulfonylfluoride | 10 mM, 50% enzyme activity inhibited | Paucimonas lemoignei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate | - |
Paucimonas lemoignei | |
0.85 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate | - |
Paucimonas lemoignei | |
1.1 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate | - |
Paucimonas lemoignei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
estimated by SDS-PAGE, deduced from sequence of cDNA | Paucimonas lemoignei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paucimonas lemoignei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydrophobic column chromatography | Paucimonas lemoignei |
Storage Stability | Organism |
---|---|
-20°C, NaCl 0.4 M, 6 months | Paucimonas lemoignei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O | - |
Paucimonas lemoignei | (R)-3-hydroxybutanoate | - |
? | |
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O | - |
Paucimonas lemoignei | (R)-3-hydroxybutanoate | - |
? | |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O | - |
Paucimonas lemoignei | (R)-3-hydroxybutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybutyrate-oligomer hydrolase | - |
Paucimonas lemoignei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Paucimonas lemoignei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate | - |
Paucimonas lemoignei | |
80 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate | - |
Paucimonas lemoignei | |
1500 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate | - |
Paucimonas lemoignei |