Cloned (Comment) | Organism |
---|---|
gene i3HBOH, DNA sequence determination and analysis, expression in Escherichia coli | Acidovorax sp. SA1 |
Protein Variants | Comment | Organism |
---|---|---|
S102A | site-directed mutagenesis, inactive mutant | Acidovorax sp. SA1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate | pH 8.0, 30°C | Acidovorax sp. SA1 | |
1.5 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate | pH 8.0, 30°C | Acidovorax sp. SA1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Acidovorax sp. SA1 | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax sp. SA1 | Q9F1T8 | - |
- |
Purification (Comment) | Organism |
---|---|
native from strain SA1 and recombinant from Escherichia coli | Acidovorax sp. SA1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O | - |
Acidovorax sp. SA1 | (R)-3-hydroxybutanoate | - |
? | |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O | - |
Acidovorax sp. SA1 | (R)-3-hydroxybutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D(-)-3-hydroxybutyrate oligomer hydrolase | - |
Acidovorax sp. SA1 |