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Literature summary for extracted from

  • Sugiyama, A.; Shiraki, M.; Kobayashi, T.; Morikawa, G.; Yamamoto, M.; Yamaoka, M.; Saito, T.
    Cloning and sequencing of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Acidovorax sp. strain SA1 and purification of the enzyme (2002), Curr. Microbiol., 45, 123-127.
    View publication on PubMed


Cloned (Comment) Organism
gene i3HBOH, DNA sequence determination and analysis, expression in Escherichia coli Acidovorax sp. SA1

Protein Variants

Protein Variants Comment Organism
S102A site-directed mutagenesis, inactive mutant Acidovorax sp. SA1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate pH 8.0, 30°C Acidovorax sp. SA1
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate pH 8.0, 30°C Acidovorax sp. SA1


Localization Comment Organism GeneOntology No. Textmining
Acidovorax sp. SA1 5622


Organism UniProt Comment Textmining
Acidovorax sp. SA1 Q9F1T8

Purification (Commentary)

Purification (Comment) Organism
native from strain SA1 and recombinant from Escherichia coli Acidovorax sp. SA1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
Acidovorax sp. SA1 (R)-3-hydroxybutanoate
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
Acidovorax sp. SA1 (R)-3-hydroxybutanoate


Synonyms Comment Organism
D(-)-3-hydroxybutyrate oligomer hydrolase
Acidovorax sp. SA1