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Literature summary for 3.1.1.22 extracted from

  • York, G.M.; Lupberger, J.; Tian, J.; Lawrence, A.G.; Stubbe, J.; Sinskey, A.J.
    Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes (2003), J. Bacteriol., 185, 3788-3794 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene phaZ1, pha sequences comparisons, subcloning in Escherichia coli strains DH5alpha and S17-1 Cupriavidus necator
gene phaZ2, pha sequences comparisons, subcloning in strains DH5alpha and S17-1 Cupriavidus necator

Engineering

Protein Variants Comment Organism
additional information generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate) Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Cupriavidus necator
-
-
additional information the enzyme sequence contains no signal sequence Cupriavidus necator
-
-

Organism

Organism UniProt Comment Textmining
Cupriavidus necator O87189 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-
Cupriavidus necator Q7WT49 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process Cupriavidus necator ?
-
?

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.1.75 Cupriavidus necator
PhaZ1
-
Cupriavidus necator
PhaZ
-
Cupriavidus necator
PhaZ2
-
Cupriavidus necator
poly[D-(-)-3-hydroxybutyrate] depolymerase
-
Cupriavidus necator
intracellular PHB depolymerase UniProt Cupriavidus necator
phaZre
-
Cupriavidus necator

General Information

General Information Comment Organism
additional information enzyme PhaZ1 has neither a lipase box nor an identifiable PHB binding domain Cupriavidus necator