Cloned (Comment) | Organism |
---|---|
gene phaZ1, pha sequences comparisons, subcloning in Escherichia coli strains DH5alpha and S17-1 | Cupriavidus necator |
gene phaZ2, pha sequences comparisons, subcloning in strains DH5alpha and S17-1 | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate) | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Cupriavidus necator | 5622 | - |
additional information | the enzyme sequence contains no signal sequence | Cupriavidus necator | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | O87189 | i.e. Alcaligenes eutrophus or Ralstonia eutropha | - |
Cupriavidus necator | Q7WT49 | i.e. Alcaligenes eutrophus or Ralstonia eutropha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process | Cupriavidus necator | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
intracellular PHB depolymerase | UniProt | Cupriavidus necator |
More | cf. EC 3.1.1.75 | Cupriavidus necator |
PhaZ | - |
Cupriavidus necator |
PhaZ1 | - |
Cupriavidus necator |
PhaZ2 | - |
Cupriavidus necator |
phaZre | - |
Cupriavidus necator |
poly[D-(-)-3-hydroxybutyrate] depolymerase | - |
Cupriavidus necator |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme PhaZ1 has neither a lipase box nor an identifiable PHB binding domain | Cupriavidus necator |