3.1.1.22: hydroxybutyrate-dimer hydrolase
This is an abbreviated version!
For detailed information about hydroxybutyrate-dimer hydrolase, go to the full flat file.
Reaction
Synonyms
3-HB-oligomer hydrolase, 3-hydroxybutyrate oligomer hydrolase, 3-hydroxybutyrate-oligomer hydrolase, 3HB oligomer hydrolase, 3HB-oligomer hydrolase, 3HBOH, D(-)-3-hydroxybutyrate oligomer hydrolase, D-(-)-3-hydroxybutyrate oligomer hydrolase, D-(-)-3-hydroxybutyrate-dimer hydrolase, H16 A2862, hydrolase, hydroxybutyrate dimer, intracellular PHB depolymerase, More, PhaZ, PhaZ1, PhaZ2, PhaZc, phaZre, poly[D-(-)-3-hydroxybutyrate] depolymerase
ECTree
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Engineering
Engineering on EC 3.1.1.22 - hydroxybutyrate-dimer hydrolase
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S191A
site-directed mutagenesis, unaltered activity compared to the wild-type enzyme
S218A
site-directed mutagenesis, slightly decreased activity compared to the wild-type enzyme
S306A
site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme
S356A
site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme
S630A
site-directed mutagenesis, increased activity compared to the wild-type enzyme
additional information
generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate)
additional information
generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate)
additional information
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generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate)
additional information
recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 (EC 3.1.1.75, UniProt ID Q0KCI0) and phaZ2 has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly
additional information
-
recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 (EC 3.1.1.75, UniProt ID Q0KCI0) and phaZ2 has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly