Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, functional expression in Escherichia coli as His-tagged enzyme, in vitro transcription and translation | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
S191A | site-directed mutagenesis, unaltered activity compared to the wild-type enzyme | Cupriavidus necator |
S218A | site-directed mutagenesis, slightly decreased activity compared to the wild-type enzyme | Cupriavidus necator |
S306A | site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme | Cupriavidus necator |
S356A | site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme | Cupriavidus necator |
S359A | site-directed mutagenesis, inactive mutant | Cupriavidus necator |
S630A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Cupriavidus necator | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, invitro transcribed and translated enzyme, SDS-PAGE | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0K9H3 | formerly Alcaligenes eutrophus, strain H16, ATCC 17699 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Cupriavidus necator |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate | Ser359 is invlved in the catalytic mechanism, active site residue | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O | - |
Cupriavidus necator | (R)-3-hydroxybutanoate | - |
? | |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O | - |
Cupriavidus necator | (R)-3-hydroxybutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, invitro transcribed and translated enzyme, SDS-PAGE | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
3HB oligomer hydrolase | - |
Cupriavidus necator |
D-(-)-3-hydroxybutyrate oligomer hydrolase | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Cupriavidus necator |