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Literature summary for 3.1.1.22 extracted from

  • Saegusa, H.; Shiraki, M.; Saito, T.
    Cloning of an intracellular D(-)-3-hydroxybutyrate-oligomer hydrolase gene from Ralstonia eutropha H16 and identification of the active site serine residue by site-directed mutagenesis (2002), J. Biosci. Bioeng., 94, 106-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, functional expression in Escherichia coli as His-tagged enzyme, in vitro transcription and translation Cupriavidus necator

Protein Variants

Protein Variants Comment Organism
S191A site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Cupriavidus necator
S218A site-directed mutagenesis, slightly decreased activity compared to the wild-type enzyme Cupriavidus necator
S306A site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme Cupriavidus necator
S356A site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme Cupriavidus necator
S359A site-directed mutagenesis, inactive mutant Cupriavidus necator
S630A site-directed mutagenesis, increased activity compared to the wild-type enzyme Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Cupriavidus necator
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 78000, invitro transcribed and translated enzyme, SDS-PAGE Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K9H3 formerly Alcaligenes eutrophus, strain H16, ATCC 17699
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
-
Cupriavidus necator (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
Cupriavidus necator (R)-3-hydroxybutanoate
-
?

Subunits

Subunits Comment Organism
? x * 78000, invitro transcribed and translated enzyme, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
D-(-)-3-hydroxybutyrate oligomer hydrolase
-
Cupriavidus necator
3HB oligomer hydrolase
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Cupriavidus necator