3.1.1.22: hydroxybutyrate-dimer hydrolase
This is an abbreviated version!
For detailed information about hydroxybutyrate-dimer hydrolase, go to the full flat file.
Word Map on EC 3.1.1.22
Reaction
+
=
2 (R)-3-hydroxybutanoate
Synonyms
3-HB-oligomer hydrolase, 3-hydroxybutyrate oligomer hydrolase, 3-hydroxybutyrate-oligomer hydrolase, 3HB oligomer hydrolase, 3HB-oligomer hydrolase, 3HBOH, D(-)-3-hydroxybutyrate oligomer hydrolase, D-(-)-3-hydroxybutyrate oligomer hydrolase, D-(-)-3-hydroxybutyrate-dimer hydrolase, H16 A2862, hydrolase, hydroxybutyrate dimer, intracellular PHB depolymerase, More, PhaZ, PhaZ1, PhaZ2, PhaZc, phaZre, poly[D-(-)-3-hydroxybutyrate] depolymerase
ECTree
Purification
Purification on EC 3.1.1.22 - hydroxybutyrate-dimer hydrolase
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2350fold, to homogeneity
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ammonium sulfate precipitation, Ni-NTA column chromatography, and TSK gel G3000SW gel filtration
hydrophobic column chromatography
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native from strain SA1 and recombinant from Escherichia coli
recombinant from Escherichia coli
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