1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.5.1.5

Reaction

5,10-methylenetetrahydrofolate
+
NADP+
=
5,10-methenyltetrahydrofolate
+
NADPH
+
H+

Synonyms

5, 10-methylenetetrahydrofolate dehydrogenase, 5,10-CH2-THF dehydrogenase, 5,10-mehtylene tetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, CH2-THF dehydrogenase, Cpe FolD, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH1, FolD, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, MIS1, mitochondrial methylenetetrahydrofolate dehydrogenase, MTHFD1, MTHFD1 protein, MTHFD2, MTHFD2 protein, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, NADP-dependent 5,10-methylene-THF dehydrogenase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.5.1.5 - methylenetetrahydrofolate dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6His-tagged enzyme is expressed in Escherichia coli
deletion of the MIS1 gene has little effect
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae strain MWY4.4 and in CHO cells
expression in Escherichia coli
-
expression in Escherichia coli BL21
gene folD, recombinant coexpression with Clostridium perfringens FchA in Escherichia coli strain K16 DELTA foldD mutants and functional complementation, overview
gene MTHFD2L, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis, the truncated enzyme mutant rMTHFD2LDELTA1-40 is cloned into vector YEp24-based yeast expression vector containing the Saccharomyces cerevisiae MET6 promoter and expressed in Saccharomyces cerevisiae strain MWY4.4 as N-terminally His6-tagged protein, replacment of the mitochondrial targeting sequence of the rat MTHFD2L with an N-terminal His tag for localization in the yeast cytosol
gene Mthfd2l, MTHFD2L is alternatively spliced at exons 2 and 8, quantitative real-time PCR enzyme expression analysis, gene expression profiling of the MTHFD gene family in mouse embryos and adult organs
hexa-His tagged DHCH1 is expressed in Escherichia coli, production of a genetic knock-out
-
in vitro transcription-translation of a cDNA clone
-
null mutation is embryonic lethal at about 12 days gestation, knockout of enzyme establishes the important role of formate production during embryogenesis
-
PCR-amplification, expressed in Escherichia coli, overexpression and null-mutant production
-
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein