1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.5.1.5

Reaction

5,10-methylenetetrahydrofolate
+
NADP+
=
5,10-methenyltetrahydrofolate
+
NADPH
+
H+

Synonyms

5, 10-methylenetetrahydrofolate dehydrogenase, 5,10-CH2-THF dehydrogenase, 5,10-mehtylene tetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, Cpe FolD, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH1, FolD, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, MIS1, mitochondrial methylenetetrahydrofolate dehydrogenase, MTHFD1, MTHFD1 protein, MTHFD2, MTHFD2 protein, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, NADP-dependent 5,10-methylene-THF dehydrogenase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+)

Storage Stability

Storage Stability on EC 1.5.1.5 - methylenetetrahydrofolate dehydrogenase (NADP+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-17C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol,
-
-20C, 0.1% bovine plasma albumin
-
-20C, 1 month, residual activity of 50%
-
-20C, stable for at least 2 months at diluted protein concentration
-
-20C, stable fot at least 2 months
-
0-4C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity
-
10% glycerol required to prevent loss of activity during storage
-
2-4C, 10% loss of activity in 4 days
-
4C, in the presence of 25% glycerol, 1 month, residual activity of 85%
-
4C, stable for at least 2 months
-
4C, stable for at least a month
-
5C, stable for several weeks in ammonium sulfate at 50% saturation
-