1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.5.1.5

Reaction

5,10-methylenetetrahydrofolate
+
NADP+
=
5,10-methenyltetrahydrofolate
+
NADPH
+
H+

Synonyms

5, 10-methylenetetrahydrofolate dehydrogenase, 5,10-CH2-THF dehydrogenase, 5,10-mehtylene tetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, Cpe FolD, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH1, FolD, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, MIS1, mitochondrial methylenetetrahydrofolate dehydrogenase, MTHFD1, MTHFD1 protein, MTHFD2, MTHFD2 protein, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, NADP-dependent 5,10-methylene-THF dehydrogenase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+)

Purification

Purification on EC 1.5.1.5 - methylenetetrahydrofolate dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase
by anion-exchange chromatography
co-purification of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methenyltetrahydrofolate cyclohydrolase
-
complex of N5,N10-methylenetetrahydrofolate dehydrogenase and N5,N10-methenyltetrahydrofolate cyclohydrolase
-
formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase and methylenetetrahydrofolate dehydrogenase combined
-
heat treatment, Ni-affinity column chromatography, and Superdex-200 gel filtration
His-Trap affinity column chromatography
-
HisTrap column chromatography and Superdex 200 gel filtration
in vitro transcription-translation of cDNA clone, trifunctional enzyme: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrase and 10-formyltetrahydrofolate synthetase
-
metal affinity chromatography; recombinant DHCH1 protein is purified by metal-affinity chromatography
-
methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined
-
partial
trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase