BRENDA - Enzyme Database
show all sequences of 1.5.1.5

The MTHFD1 p.Arg653Gln variant alters enzyme function and increases risk for congenital heart defects

Christensen, K.E.; Rohlicek, C.V.; Andelfinger, G.U.; Michaud, J.; Bigras, J.L.; Richter, A.; Mackenzie, R.E.; Rozen, R.; Hum. Mutat. 30, 212-220 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
R653Q
mutant enzyme has normal substrate affinity but a 36% reduction in half-life at 42°C
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5,10-methylene tetrahydrofolate + NADP+
Homo sapiens
dehydrogenase activity of MTHFD1
5,10-methenyltetrahydrofolate + NADPH
-
-
r
additional information
Homo sapiens
(MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
P11586
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by anion-exchange chromatography
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.2
-
dehydrogenase activity of the wild type enzyme
Homo sapiens
13.9
-
dehydrogenase activity of the mutant R653Q
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5,10-methylene tetrahydrofolate + NADP+
dehydrogenase activity of MTHFD1
698187
Homo sapiens
5,10-methenyltetrahydrofolate + NADPH
-
-
-
r
additional information
(MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis
698187
Homo sapiens
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
methylenetetrahydrofolate dehydrogenase
-
Homo sapiens
methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase
-
Homo sapiens
MTHFD1
-
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
R653Q
mutant enzyme has normal substrate affinity but a 36% reduction in half-life at 42°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5,10-methylene tetrahydrofolate + NADP+
Homo sapiens
dehydrogenase activity of MTHFD1
5,10-methenyltetrahydrofolate + NADPH
-
-
r
additional information
Homo sapiens
(MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
by anion-exchange chromatography
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.2
-
dehydrogenase activity of the wild type enzyme
Homo sapiens
13.9
-
dehydrogenase activity of the mutant R653Q
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5,10-methylene tetrahydrofolate + NADP+
dehydrogenase activity of MTHFD1
698187
Homo sapiens
5,10-methenyltetrahydrofolate + NADPH
-
-
-
r
additional information
(MTHFD1) is a trifunctional enzyme that interconverts tetrahydrofolate derivatives for nucleotide synthesis
698187
Homo sapiens
?
-
-
-
-
Other publictions for EC 1.5.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743250
Aluri
Physiological role of FolD (m ...
Clostridium perfringens
Microbiology
162
145-155
2016
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1
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1
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2
2
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-
720406
Eadsforth
The crystal structure of Leish ...
Leishmania major, Leishmania major Fridlin
Mol. Biochem. Parasitol.
181
178-185
2012
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1
1
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1
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2
2
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2
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1
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2
1
2
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1
1
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1
1
1
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1
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2
2
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2
1
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718743
Lee
Crystal structure of bifunctio ...
Thermoplasma acidophilum
Biochem. Biophys. Res. Commun.
406
459-463
2011
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1
1
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1
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1
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1
1
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725456
Bolusani
Mammalian MTHFD2L encodes a mi ...
Homo sapiens, Rattus norvegicus
J. Biol. Chem.
286
5166-5174
2011
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-
2
-
-
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2
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2
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6
-
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12
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2
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4
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2
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2
2
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2
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2
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12
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2
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-
-
712807
Bi
Association analysis of CbetaS ...
Homo sapiens
J. Neural Transm.
117
499-503
2010
-
1
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1
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698187
Christensen
The MTHFD1 p.Arg653Gln variant ...
Homo sapiens
Hum. Mutat.
30
212-220
2009
-
-
1
-
1
-
-
-
-
-
-
2
-
1
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1
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2
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2
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3
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1
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2
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2
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-
700281
Murta
Methylene tetrahydrofolate deh ...
Leishmania major
Mol. Microbiol.
71
1386-1401
2009
-
1
1
-
1
-
7
2
1
-
-
3
-
1
-
-
1
-
-
-
1
1
3
-
4
1
-
-
3
1
-
-
1
2
-
14
-
1
1
1
-
1
-
14
7
2
2
1
-
-
3
-
-
-
1
-
-
1
1
3
-
1
-
-
3
1
-
-
-
-
1
1
-
2
2
688323
Kim
Crystallization and preliminar ...
Thermoplasma acidophilum
J. Microbiol. Biotechnol.
18
283-286
2008
-
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1
1
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-
-
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1
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1
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1
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1
1
2
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2
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1
2
1
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1
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1
1
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-
701402
Christensen
Mitochondrial methylenetetrahy ...
Homo sapiens, Saccharomyces cerevisiae
Vitam. Horm.
79
393-410
2008
-
-
2
-
3
-
1
1
4
3
1
7
-
2
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2
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7
-
11
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3
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2
3
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3
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1
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1
4
3
1
7
-
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2
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-
7
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
654937
Casaite
-
Elucidation of enzymatic funct ...
Arthrobacter globiformis
Biologia (Bratisl. )
2
1-4
2004
-
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1
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1
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1
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1
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1
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1
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-
-
-
-
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-
-
-
-
392334
Goenrich
Purification and characterizat ...
Hyphomicrobium zavarzinii, Hyphomicrobium zavarzinii ZV580, no activity in Methylobacterium extorquens
Arch. Microbiol.
177
299-303
2002
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2
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1
2
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4
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1
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1
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2
1
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1
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1
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1
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2
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1
2
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1
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1
-
2
1
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1
-
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-
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392333
Pawelek
Methenyltetrahydrofolate cyclo ...
Homo sapiens, Photobacterium phosphoreum
Biochemistry
37
1109-1115
1998
-
-
-
-
-
-
-
7
-
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1
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2
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2
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4
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2
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2
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7
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1
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2
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4
-
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-
209738
Pawelek
Methylenetetrahydrofolate dehy ...
Photobacterium phosphoreum
Biochim. Biophys. Acta
1296
47-54
1996
1
-
1
-
-
-
2
4
-
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1
1
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1
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1
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1
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1
1
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2
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2
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1
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1
2
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2
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4
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1
1
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1
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1
-
1
1
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-
2
-
-
-
-
-
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-
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-
209737
Kirk
-
A 5,10-methylenetetrahydrofola ...
Pisum sativum
Phytochemistry
39
1309-1317
1995
-
-
-
-
-
-
4
3
-
-
2
-
-
1
-
-
1
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-
2
1
2
2
1
-
1
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-
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1
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1
3
-
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1
-
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4
3
3
-
-
2
-
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1
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2
1
2
2
1
1
-
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1
-
-
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392331
Pelletier J.N.; MacKenzie
Binding and interconversion of ...
Homo sapiens
Biochemistry
34
12673-12680
1995
-
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1
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1
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1518
Cossins
Enzymes for synthesis of 10-fo ...
Pisum sativum
Adv. Exp. Med. Biol.
338
707-710
1993
-
-
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-
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2
1
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2
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1
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1
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1
1
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1
1
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1
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1
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1
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2
1
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2
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1
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1
1
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1
1
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1
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-
392079
Mackenzie
Methylenetetrahydrofolate dehy ...
Homo sapiens, Mus musculus
Adv. Enzyme Regul.
27
31-39
1988
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5
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5
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392315
Hum
Primary structure of a human t ...
Homo sapiens
J. Biol. Chem.
263
15946-15950
1988
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1
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392323
Gardam
The NADP-dependent trifunction ...
Mus musculus
Biochem. Cell Biol.
66
66-70
1988
-
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2
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1
-
1
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1
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1
1
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1
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1
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1
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1
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1
1
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1
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1532
Villar
C1-Tetrahydrofolate synthase f ...
Oryctolagus cuniculus
J. Biol. Chem.
260
2245-2252
1985
-
-
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2
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1
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2
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1
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1
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1
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1
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1
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392321
Ross
Studies on the polyglutamate s ...
Sus scrofa
Biochemistry
23
1796-1801
1984
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1
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1
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1
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1505
Paukert
Formyl-methenyl-methylenetetra ...
Ovis aries, Saccharomyces cerevisiae, Sus scrofa
Methods Enzymol.
66
616-626
1980
-
-
-
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2
6
-
1
4
3
-
3
-
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3
-
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2
1
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3
1
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3
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3
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2
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6
-
1
4
3
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-
-
3
-
2
1
-
3
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
392309
Ljungdahl
Methylenetetrahydrofolate dehy ...
Moorella thermoacetica
Methods Enzymol.
66
599-609
1980
-
-
-
-
-
1
-
1
-
-
2
1
-
1
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1
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-
-
2
1
1
1
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1
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1
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1
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1
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1
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1
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2
1
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-
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1
-
-
2
1
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
392317
MacKenzie
Methylenetetrahydrofolate dehy ...
Sus scrofa
Methods Enzymol.
66
609-615
1980
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
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1
-
-
1
1
1
1
1
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-
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1
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1
-
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1
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2
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1
-
-
-
-
1
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209726
Cohen
Methylenetetrahydrofolate dehy ...
Sus scrofa
Biochim. Biophys. Acta
522
311-317
1978
-
-
-
-
-
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2
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-
-
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2
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1
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2
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2
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1
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2
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-
-
392307
Dev
A complex of N5,N10-methylenet ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
253
4245-4253
1978
-
-
-
-
-
-
3
-
-
-
-
-
-
178
-
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1
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-
1
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-
-
1
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1
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-
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3
1
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1
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1
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-
1
-
-
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-
-
-
-
-
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-
-
-
-
209727
Tan
Methylenetetrahydrofolate dehy ...
Sus scrofa
J. Biol. Chem.
252
1117-1122
1977
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-
-
-
-
1
3
2
-
-
2
1
-
1
-
-
1
-
-
1
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-
1
1
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-
-
-
-
-
-
-
1
-
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-
-
-
-
1
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-
1
-
3
-
2
-
-
2
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392314
Paukert
Formyl-methenyl-methylenetetra ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
77
147-154
1977
-
-
-
-
-
-
-
-
-
-
2
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1
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1
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1
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2
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1
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-
-
1
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-
-
-
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-
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-
-
-
392324
Paukert
Formyl-methyl-methylenetetrahy ...
Ovis aries
J. Biol. Chem.
251
5104-5111
1976
-
-
-
-
-
-
-
2
-
-
3
1
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1
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1
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1
1
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1
1
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1
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-
1
-
-
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-
-
2
-
-
3
1
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392308
Harvey
Regulation in the folate pathw ...
Escherichia coli, Escherichia coli B / ATCC 11303
Adv. Enzyme Regul.
13
99-124
1975
-
-
-
-
-
-
3
-
-
-
-
2
-
177
-
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2
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3
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2
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-
-
2
-
-
-
-
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-
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-
-
-
392311
O'Brien
Purification and characterizat ...
Moorella thermoacetica
J. Biol. Chem.
248
403-408
1973
-
-
-
-
-
-
1
2
-
-
1
1
-
2
-
-
1
-
-
-
1
2
1
1
-
1
-
1
-
-
-
-
1
1
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-
-
1
-
-
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-
1
1
2
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-
1
1
-
-
-
1
-
-
1
2
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
392319
MacKenzie
Co-purification of three folat ...
Sus scrofa
Biochem. Biophys. Res. Commun.
53
1088-1095
1973
-
-
-
-
-
1
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
392328
Yoshida
Comparative study on major pat ...
Bos taurus, Elaphe quadrivirgata, Gallus gallus bankiva, Ovis aries
J. Biochem.
72
1503-1516
1972
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-
-
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8
-
-
4
-
4
-
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-
4
8
-
4
-
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-
-
8
-
-
4
-
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-
4
8
-
4
-
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-
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-
-
-
-
-
-
-
-
-
392326
Cossins
-
Plant N5,N10-methylenetetrahyd ...
Pisum sativum
Phytochemistry
9
1463-1471
1970
-
-
-
-
-
1
3
3
-
-
-
-
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1
-
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1
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-
3
1
1
2
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
1
-
3
-
3
-
-
-
-
-
-
-
1
-
3
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
392306
Albrecht
Methylenetetrahydrofolate dehy ...
Enterococcus faecium
J. Bacteriol.
95
1779-1789
1968
-
-
-
-
-
1
5
2
-
-
-
1
-
1
-
-
1
-
-
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-
1
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
-
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1
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
392310
Uyeda
Enzymes of clostridial purine ...
Clostridium cylindrosporum
J. Biol. Chem.
242
4378-4385
1967
-
-
-
-
-
1
1
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
-
1
1
-
-
-
1
-
-
1
1
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
392312
Dalal
Purification of 5,10-methylene ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
242
3636-3640
1967
-
-
-
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-
1
3
2
-
-
-
2
-
44
-
-
1
-
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-
2
1
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
1
-
-
1
-
3
1
2
-
-
-
2
-
-
-
1
-
-
2
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392316
Yeh
Purification and properties of ...
Bos taurus
Biochim. Biophys. Acta
105
279-291
1965
-
-
-
-
-
1
6
3
-
1
-
1
-
1
-
-
-
-
-
1
-
1
3
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
6
-
3
-
1
-
1
-
-
-
-
-
1
-
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
392313
Ramasastri
5,10-Methylenetetrahydrofolic ...
Saccharomyces cerevisiae
J. Biol. Chem.
237
1982-1988
1962
5
-
-
-
-
1
5
2
-
1
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
1
-
-
1
-
-
-
5
-
-
1
-
-
1
-
5
-
2
-
1
-
1
-
-
-
1
-
-
1
-
1
-
1
-
1
-
1
-
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-
-