1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.5.1.5

Reaction

5,10-methylenetetrahydrofolate
+
NADP+
=
5,10-methenyltetrahydrofolate
+
NADPH
+
H+

Synonyms

5, 10-methylenetetrahydrofolate dehydrogenase, 5,10-CH2-THF dehydrogenase, 5,10-mehtylene tetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, Cpe FolD, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH1, FolD, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, MIS1, mitochondrial methylenetetrahydrofolate dehydrogenase, MTHFD1, MTHFD1 protein, MTHFD2, MTHFD2 protein, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, NADP-dependent 5,10-methylene-THF dehydrogenase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+)

Specific Activity

Specific Activity on EC 1.5.1.5 - methylenetetrahydrofolate dehydrogenase (NADP+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.009
-
mitochondria
0.011
-
mitochondria
0.028
-
mitochondria
0.029
-
mitochondria
0.038
-
soluble
0.05
-
soluble
0.092
-
soluble
0.164
-
soluble
12.7 - 42
-
trifunctional NADP+-dependent enzyme, in various cell lines
13.2
dehydrogenase activity of the wild type enzyme
13.9
dehydrogenase activity of the mutant R653Q
2.4 - 6.9
-
bifunctional NAD+-dependent enzyme, in various cell lines
2.7 - 12.9
-
bifunctional NAD+-dependent enzyme, in various cell lines
22
-
25 mM MOPS pH 7.3, 2.5 mM formaldehyde, 1 mM NADP+, 30 mM 2-mercaptoethanol, 27°C, 6.3 microM/min/mg activity of the methenyltetrahydrofolate cyclohydrolase; dehydrogenase activity is measured by following the conversion of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate
310
-
assays at 37°C
32.5
-
-
6.53
-
-
720
-
at 60°C growth temperature
8 - 17.9
-
trifuncional NADP+-dependent enzyme, in various cell lines
8.1
-
trifunctional enzyme, human liver
97.4
-
recombinant bifunctional enzyme
additional information