1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
 +
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoS, ChOx, CO, CO1, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Temperature Stability

Temperature Stability on EC 1.1.3.6 - cholesterol oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 40
-
purified enzyme, stable at
37
-
purified enzyme, half-life is about 2 h
4 - 50
-
strain ST-200, 27% activity lost after incubation at 60°C, complete loss of activity at 70°C
4 - 65
40 - 50
-
purified enzyme, about 80% reduction of activity after 100 days
40 - 60
the enzyme is stable below 40°C without obvious inactivation after 6 h of incubation. When the temperature exceeds 50°C, the ChoL activity rapidly decreases. The enzyme retains approximately 40% of its initial activity after incubation for 6 h at 50°C and 17% after incubation for 1 h at 60°C
45 - 55
after 1 h of incubation, more than 70 and 50% of activity remains at 45 and 55°C, respectively
50 - 60
Corynebacterium cholesterolicum
-
96% activity after 30 min at 50°C, 6% activity after 30 min at 60°C
60 - 70
-
purified enzyme, activity s reduced to 85% and 48% after 10 days and is rapidly reduced during further incubation
60 - 85
-
the enzyme retains more than 90% of its original activity after incubation for 240 min at 60°C, 90% activity after 1 h at 80°C, and 80% activity after 30 min at 85°C
65
-
purified enzyme, 30 min, 46% activity remaining
70 - 80
75
-
purified enzyme, 30 min, 14% activity remaining
75 - 80
purified recombinant enzyme, pH 7.0, 30 min, inactivation
85
-
30 min, 20% loss of activity
90
-
30 min, 72% loss of activity