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Literature summary for 1.1.3.6 extracted from
- Purification and characterization of an extracellular cholesterol oxidase of Bacillus subtilis isolated from Tiger excreta (2016), Appl. Biochem. Biotechnol., 178, 353-367 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the bacterial COX exhibits 22 % higher activity in potassium phosphate buffer, pH 7.5, in the presence of a nonionic detergent Triton X-100 at 0.05 % v/v | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus subtilis | |
| 3.25 | - |
cholesterol | pH 7.5, 37°C | Bacillus subtilis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
native PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | Bacillus subtilis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isolated from tiger excreta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 28.4fold from cell culture supernatant by ammonium sulfate fractionation, dialysis, and riboflavin affinity chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.39 | - |
purified enzyme, pH 7.5, 37°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | - |
Bacillus subtilis | cholest-4-en-3-one + H2O2 | - |
? | |
| additional information | optimization of assay conditions, concerning buffer type and molarity, temperature, pH, deergent addition, ionogenic state etc. | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * 21500, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| COX | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | activity range, profile overview | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified enzyme, half-life is about 2 h | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | activity range, profile overview | Bacillus subtilis |
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