Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sarcosyl | activates at 0.5% w/v | Pseudomonas aeruginosa | |
sodium cholate | activates at 0.5% w/v | Pseudomonas aeruginosa | |
Toluene | 12% activation in a ration of 1:2 | Pseudomonas aeruginosa | |
Triton X-100 | activates at 0.5% w/v | Pseudomonas aeruginosa | |
Triton X-405 | activates at 0.5% w/v | Pseudomonas aeruginosa | |
Tween 20 | activates at 0.5% w/v | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene choP, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS in inclusion bodies from cells grown at 20°C and 30°C. A soluble and active PA157 enzyme is obtained when the recombinant cells were grown at 10°C | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | complete inhibition | Pseudomonas aeruginosa | |
sodium laurylbenzenesulfonate | complete inhibition | Pseudomonas aeruginosa | |
sodium polyoxyethylene alkyl ether sulfate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0926 | - |
cholesterol | pH 7.0-8.0, 30°C | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 1 mM of Ca2+, Mg2+, Mn2+, Ni2+, Fe2+, and Zn2+ have no obvious effect on the enzyme activity.No effect by EDTA | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Pseudomonas aeruginosa | - |
6beta-hydroxycholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Pseudomonas aeruginosa PA157 | - |
6beta-hydroxycholest-4-en-3-one + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 2% activity remaining | Pseudomonas aeruginosa |
chloroform | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 89% activity remaining | Pseudomonas aeruginosa |
cyclooctane | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 108% activity remaining | Pseudomonas aeruginosa |
Ethanol | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 31% activity remaining | Pseudomonas aeruginosa |
isopropanol | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 6% activity remaining | Pseudomonas aeruginosa |
Methanol | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 65% activity remaining | Pseudomonas aeruginosa |
p-xylene | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 102% activity remaining | Pseudomonas aeruginosa |
toluene | in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 112% activity remaining | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | X5IDD2 | - |
- |
Pseudomonas aeruginosa PA157 | X5IDD2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-betacholestanol + O2 | - |
Pseudomonas aeruginosa | ? + H2O2 | - |
? | |
3-betacholestanol + O2 | - |
Pseudomonas aeruginosa PA157 | ? + H2O2 | - |
? | |
beta-sitosterol + O2 | - |
Pseudomonas aeruginosa | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | - |
Pseudomonas aeruginosa PA157 | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | - |
Pseudomonas aeruginosa | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | - |
Pseudomonas aeruginosa PA157 | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas aeruginosa | 6beta-hydroxycholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas aeruginosa PA157 | 6beta-hydroxycholest-4-en-3-one + H2O2 | - |
? | |
additional information | the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one | Pseudomonas aeruginosa PA157 | ? | - |
? | |
pregnenolone + O2 | low activity | Pseudomonas aeruginosa | pregn-5-ene-3,20-dione + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65344, mass spectrometric analysis | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
choP | - |
Pseudomonas aeruginosa |
HCEO-forming enzyme | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 65 | purified recombinant enzyme, pH 7.0, 30 min, stable at | Pseudomonas aeruginosa |
70 | - |
purified recombinant enzyme, pH 7.0, 30 min, 90% activity remaining | Pseudomonas aeruginosa |
75 | 80 | purified recombinant enzyme, pH 7.0, 30 min, inactivation | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | purified recombinant enzyme, 30°C, 10% activity remaining after 30 min | Pseudomonas aeruginosa |
5.5 | 10 | purified recombinant enzyme, 30°C, 30 min, stable at | Pseudomonas aeruginosa |
11 | - |
purified recombinant enzyme, 30°C, 30 min, 70% activity remaining | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.96 |