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Literature summary for 1.1.3.6 extracted from

  • Fujishiro, K.; Uchida, H.; Shimokawa, K.; Nakano, M.; Sano, F.; Ohta, T.; Kayahara, N.; Aisaka, K.; Uwajima, T.
    Purification and properties of a new Brevibacterium sterolicum cholesterol oxidase produced by E. coli MM294/pnH10 (2002), FEMS Microbiol. Lett., 215, 243-248.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cholesterol oxidase II, expression in Escherichia coli MM294/pnH10 Brevibacterium sterolicum

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 cholesterol oxidase II Brevibacterium sterolicum
HgCl2 cholesterol oxidase II Brevibacterium sterolicum
PCMB cholesterol oxidase II Brevibacterium sterolicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
cholesterol pH 7.0, 37°C, cholesterol oxidase II Brevibacterium sterolicum
1.1
-
cholesterol pH 7.0, 37°C, cholesterol oxidase I Brevibacterium sterolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
cholesterol oxidase II, gel filtration Brevibacterium sterolicum

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
nov. sp. ATCC21387
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevibacterium sterolicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55.2
-
cholesterol oxidase II Brevibacterium sterolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-sitosterol + O2 about 30% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum stigmast-5-en-3-one + H2O2
-
?
beta-sitosterol + O2 about 55% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
?
cholesterol + O2
-
Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
?
dehydro-epi-androsterone + O2 about 35% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum ?
-
?
dehydro-epi-androsterone + O2 about 5% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
?
pregnenolone + O2 about 60% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum ?
-
?
pregnenolone + O2 about 60% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
?
stigmasterol + O2 about 10% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
?
stigmasterol + O2 about 10% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
?

Synonyms

Synonyms Comment Organism
cholesterol oxidase I
-
Brevibacterium sterolicum
cholesterol oxidase II
-
Brevibacterium sterolicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
cholesterol oxidase II Brevibacterium sterolicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.0, 1 h, 10% loss of activity Brevibacterium sterolicum
60
-
pH 7.0, 1 h, 82% loss of activity Brevibacterium sterolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
cholesterol oxidase II Brevibacterium sterolicum
7
-
cholesterol oxidase I Brevibacterium sterolicum

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 75% of maximalm acttivity, pH 8.5: about 60% of maximal activity, cholesterol oxidase II Brevibacterium sterolicum
5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, cholesterol oxidase I Brevibacterium sterolicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 50°C, 1 h, stable Brevibacterium sterolicum

Cofactor

Cofactor Comment Organism Structure
flavin
-
Brevibacterium sterolicum

pI Value

Organism Comment pI Value Maximum pI Value
Brevibacterium sterolicum isoelectric focusing with carrier ampholites pH 4-6, cholesterol oxidase II
-
4.9