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Literature summary for 1.1.3.6 extracted from

  • Isobe, K.; Shoji, K.; Nakanishi, Y.; Yokoe, M.; Wakao, N.
    Purification and some properties of cholesterol oxidase stable in detergents from gamma-Proteobacterium Y-134 (2003), J. Biosci. Bioeng., 95, 257-263.
    View publication on PubMed
Search for more literature for 1.1.3.6

General Stability

General Stability Organism
stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, pH 7.0, at concentrations of 0.1% Emulgen A-60, Triton X-100, Triton X-405 or sodium cholate gamma proteobacterium Y-134

Inhibitors

Inhibitors Comment Organism Structure
2-pyridylhydroxymethane sulfate 1 mM, 30 min at 4°C, 34% inhibition gamma proteobacterium Y-134
8-hydroxyquinoline 1 mM, 30 min at 4°C, 29% inhibition gamma proteobacterium Y-134
FAD 1 mM, 30 min at 4°C, 9% inhibition gamma proteobacterium Y-134
FMN 1 mM, 30 min at 4°C, 11% inhibition gamma proteobacterium Y-134
o-phenanthroline 1 mM, 30 min at 4°C, 30% inhibition gamma proteobacterium Y-134
potassium cyanide 1 mM, 30 min at 4°C, 12% inhibition gamma proteobacterium Y-134

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 1 mM, 30 min at 4°C, activation to 113% of control gamma proteobacterium Y-134
MnCl2 1 mM, 30 min at 4°C, 35% inhibition gamma proteobacterium Y-134

Organism

Organism UniProt Comment Textmining
gamma proteobacterium Y-134
-
 uncultured
-

Purification (Commentary)

Purification (Comment) Organism
-
 gamma proteobacterium Y-134

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme production reaches its maximum by incubation at 30°C for 12 d gamma proteobacterium Y-134
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.4
-
-
 gamma proteobacterium Y-134

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-dehydrocholesterol + O2 14% of the activity with cholesterol gamma proteobacterium Y-134 cholest-4,7-dien-3-one + H2O2
-
 ?
beta-sitosterol + O2 29% of the activity with cholesterol gamma proteobacterium Y-134 stigmast-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 gamma proteobacterium Y-134 cholest-4-en-3-one + H2O2
-
 ?
dihydrocholesterol + O2 79% of the activity with cholesterol gamma proteobacterium Y-134 5alpha-cholestan-3-one + H2O2
-
 ?
additional information no activity with: ergosterol, dehydroepiandrosterone, epiandrosterone, pregnenolone gamma proteobacterium Y-134 ?
-
 ?
stigmastanol + O2 15% of the activity with cholesterol gamma proteobacterium Y-134 stigmastanone + H2O2
-
 ?
stigmasterol + O2 32% of the activity with cholesterol gamma proteobacterium Y-134 stigmasta-5,22-dien-3-one + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 gamma proteobacterium Y-134

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 85% of maximal activity, 70°C: about 80% of maximal activity gamma proteobacterium Y-134

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7.0, 60 min, about 10% loss of activity gamma proteobacterium Y-134
70
-
 pH 7.0, 10 min, about 55% loss of activity gamma proteobacterium Y-134

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 gamma proteobacterium Y-134

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 70% of maximal activity, pH 7.5: about 30% of maximal activity gamma proteobacterium Y-134

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6.5 60°C, 60 min, stable gamma proteobacterium Y-134
7.5
-
 60°C, 60 min, about 10% loss of activity gamma proteobacterium Y-134
8
-
 60°C, 60 min, about 40% loss of activity gamma proteobacterium Y-134

pI Value

Organism Comment pI Value Maximum pI Value
gamma proteobacterium Y-134 isoelectric focusing, pH-gradient 3.5-10.0
-
 4.2