Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzene | 9% activation at 0.5% w/v | Rhodococcus sp. | |
chloroform | 7% activation at 0.5% w/v | Rhodococcus sp. | |
ethanol | 10% activation at 0.5% w/v | Rhodococcus sp. | |
Isopropanol | 15% activation at 0.5% w/v | Rhodococcus sp. | |
NaN3 | activates slightly | Rhodococcus sp. | |
Tween-80 | 18% activation at 0.5% w/v | Rhodococcus sp. |
General Stability | Organism |
---|---|
purified enzyme, 80% residual activity with 20% NaCl (3.42 M) and 83% relative activity with 12% NaCl (2.05 M) concentration. The enzyme is stable in presence of chemical reagents (NaN3, EDTA), detergents (Tween-80, Tween-20, Triton X-100, sodium cholate) | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 85% inhibition at 0.1 mM | Rhodococcus sp. | |
acetone | 85% inhibition at a ratio of 1:2 with enzyme solution | Rhodococcus sp. | |
Ag+ | 75% inhibition at 0.1 mM | Rhodococcus sp. | |
Ba2+ | 91% inhibition at 0.1 mM | Rhodococcus sp. | |
butanol | 6% inhibition at a ratio of 1:2 with enzyme solution | Rhodococcus sp. | |
Ca2+ | - |
Rhodococcus sp. | |
Cu2+ | 69% inhibition at 0.1 mM | Rhodococcus sp. | |
DMSO | 11% inhibition at a ratio of 1:2 with enzyme solution | Rhodococcus sp. | |
Fe2+ | 10% inhibition at 0.1 mM | Rhodococcus sp. | |
Fe3+ | 88% inhibition at 0.1 mM | Rhodococcus sp. | |
Hg2+ | complete inhibition at 0.1 mM | Rhodococcus sp. | |
Li+ | 8% inhibition at 0.1 mM | Rhodococcus sp. | |
Mg2+ | - |
Rhodococcus sp. | |
additional information | the enzyme activity is unaffected or poorly affected by Tween-20, Triton X-100, sodium cholate, methanol, toluene, and ethyl acetate | Rhodococcus sp. | |
NaCl | 80% residual activity with 20% NaCl (3.42 M) and 83% relative activity with 12% NaCl (2.05 M) concentration | Rhodococcus sp. | |
SDS | 88% inhibition at 0.5% w/v | Rhodococcus sp. | |
Zn2+ | 67% inhibition at 0.1 mM | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
cholesterol | pH 8.0, 37°C | Rhodococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhodococcus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is unaffected or poorly affected by Ca2+, Mg2+, Mn2+, and EDTA | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Rhodococcus sp. | - |
cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Rhodococcus sp. PKPD-CL | - |
cholest-4-en-3-one + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
benzene | stable in presence of | Rhodococcus sp. |
Butanol | stable in presence of | Rhodococcus sp. |
chloroform | stable in presence of | Rhodococcus sp. |
dimethyl sulfoxide | stable in presence of | Rhodococcus sp. |
Ethanol | stable in presence of | Rhodococcus sp. |
Ethyl acetate | stable in presence of | Rhodococcus sp. |
isopropanol | stable in presence of | Rhodococcus sp. |
Methanol | stable in presence of | Rhodococcus sp. |
toluene | stable in presence of | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
isolated from brackish water of the Chilika Lake at Odisha state of India | - |
Rhodococcus sp. PKPD-CL | - |
isolated from brackish water of the Chilika Lake at Odisha state of India | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme 59fold from strain PKPD-CL by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.64 | - |
purified enzyme, pH 8.0, 37°C | Rhodococcus sp. |
Storage Stability | Organism |
---|---|
30°C, purified enzyme, completely stable for 60 days, 24% reduction after 70 days, 60% reduction after 100 days | Rhodococcus sp. |
4°C, purified enzyme, completely stable for 60 days, 24% reduction after 70 days, 60% reduction after 100 days | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + O2 | 85% activity compared to cholesterol | Rhodococcus sp. | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 85% activity compared to cholesterol | Rhodococcus sp. PKPD-CL | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | 83% activity compared to cholesterol | Rhodococcus sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | 83% activity compared to cholesterol | Rhodococcus sp. PKPD-CL | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
cholestenol + O2 | 43% activity compared to cholesterol | Rhodococcus sp. | 5alpha-cholest-8-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Rhodococcus sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | best substrate | Rhodococcus sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Rhodococcus sp. PKPD-CL | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | best substrate | Rhodococcus sp. PKPD-CL | cholest-4-en-3-one + H2O2 | - |
? | |
ergosterol + O2 | 22% activity compared to cholesterol | Rhodococcus sp. | ? + H2O2 | - |
? | |
additional information | the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate | Rhodococcus sp. | ? | - |
? | |
additional information | the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate | Rhodococcus sp. PKPD-CL | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Rhodococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
ChOx | - |
Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rhodococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | activity range, profile overview | Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | purified enzyme, about 80% reduction of activity after 100 days | Rhodococcus sp. |
60 | 70 | purified enzyme, activity s reduced to 85% and 48% after 10 days and is rapidly reduced during further incubation | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 13 | activity range, profile overview | Rhodococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
purified enzyme, loss of 80% activity | Rhodococcus sp. |
6 | 8.5 | purified enzyme, completely stable at | Rhodococcus sp. |
9.5 | - |
purified enzyme, loss of 85% activity | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus sp. | isoelectric focusing | - |
9.5 |
General Information | Comment | Organism |
---|---|---|
additional information | a extracellular halo tolerant, detergent and organic solvent stable alkaline cholesterol oxidase. The enzyme is stable in presence of chemical reagents (NaN3, EDTA), detergents (Tween-80, Tween-20, Triton X-100, sodium cholate) and various organic solvents (isopropanol, ethanol, benzene, chloroform, methanol, toluene, ethyl acetate, butanol and dimethylsulfoxide) | Rhodococcus sp. |