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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

General Stability

General Stability on EC 1.1.3.6 - cholesterol oxidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
65% activity remains after 24 h at 25°C in 50 mM potassium phosphate, pH 7.5, enzyme is completely stable for 300 min in the presence of 1% propan-2-ol and 1% thesit as well as in the presence of 10% propan-2-ol and 8% thesit
after being reused six times, the enzyme immobilized on polyacrylonitrile hollow fibers retains more than 80% of its activity
-
bile salts stabilize
-
long-chain primary alcohols stabilize
-
purified enzyme, 80% residual activity with 20% NaCl (3.42 M) and 83% relative activity with 12% NaCl (2.05 M) concentration. The enzyme is stable in presence of chemical reagents (NaN3, EDTA), detergents (Tween-80, Tween-20, Triton X-100, sodium cholate)
-
SDS, 0.2 mM, stabilizes
-
stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, at concentrations of 0.1% and 0.5% of emulgen A-60, Triton X-100, Triton X-405 or sodium cholate
-
stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, pH 7.0, at concentrations of 0.1% Emulgen A-60, Triton X-100, Triton X-405 or sodium cholate
-
the ChOx-immobilized silk mat maintains its initial activity till the fourth successive measurement and retains about 50% of its initial activity at the end of 25 measurements
-
thermostability of the enzyme immobilized and encapsulated as Cu-COD hybrid nanoflowers is significantly enhanced