1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
 +
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoS, ChOx, CO, CO1, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

pH Range

pH Range on EC 1.1.3.6 - cholesterol oxidase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 13
-
activity range, profile overview
4 - 10
4 - 7.5
-
the activity increases with increasing pH value from 4.0 to 7.5 and then declines as pH increases further
4 - 8
-
pH 4.0: native enzyme showes about 40% of maximal activity, enzyme immobilized on polyacrylonitrile hollow fibers shows about 65% of maximal activity, pH 8.0: native enzyme showes about 60% of maximal activity, enzyme immobilized on polyacrylonitrile hollow fibers shows about 85% of maximal activity
4.5 - 8
Nocardia rhodochrous
-
about 50% of maximal activity at pH 4.5, about 90% of maximal activity at pH 8
5 - 11
-
activity range
5 - 6.5
-
pH 5.0: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, pH 7.0: about 20% of maximal activity
5 - 7.5
-
pH 5.0: about 70% of maximal activity, pH 7.5: about 30% of maximal activity
5 - 8
-
pH 5: 78% of maximal activity, pH 8.0: 75% of maximal activity, reaction with cholestero, enzyme CO2
5 - 8.5
5 - 9
5 - 9.5
-
about 50% of maximal activity at pH 5 and 9.5
5.5 - 8
relative high activities in a broad pH range of 6.0-8.0, however activity rapidly declines under pH 5.5
5.5 - 8.5
-
pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, cholesterol oxidase I
6 - 8
-
wild-type, S103T, V121A, R135H and V145E mutants
6 - 9.5
6.5 - 8.5
-
activity range, profile overview
6.5 - 9
additional information
-
pH-dependencies of native and recombinant non-tagged or His-tagged enzyme is similar, overview