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Literature summary for 1.1.3.6 extracted from

  • Fazaeli, A.; Golestani, A.; Lakzaei, M.; Rasi Varaei, S.S.; Aminian, M.
    Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization (2018), AMB Express, 8, 183 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Applying the optimized protocol, the production of recombinant cholesterol oxidase is significantly enhanced from 3.2 to 158 U/l. Under the optimized condition, the enzyme is produced on a large-scale Streptomyces sp. SA-COO

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 cholesterol pH 7, 25°C Streptomyces sp. SA-COO

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Streptomyces sp. SA-COO
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. SA-COO P12676
-
-

Purification (Commentary)

Purification (Comment) Organism
column nickel affinity chromatography Streptomyces sp. SA-COO

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Streptomyces sp. SA-COO cholest-5-en-3-one + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
ChoA
-
 Streptomyces sp. SA-COO

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp. SA-COO

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 42% of maximal activity, 80°C: about 24% of maximal activity Streptomyces sp. SA-COO
40 70 the enzyme activity retains more than 60% of its activity at the temperatures from 40°C to 70°C Streptomyces sp. SA-COO

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 the enzyme activity retains more than 95% of its maximal activity within the pH range of 6-7 at 25°C Streptomyces sp. SA-COO

pH Range

pH Minimum pH Maximum Comment Organism
6 7 the enzyme activity retains more than 95% of its maximal activity within the pH range of 6-7 at 25°C Streptomyces sp. SA-COO