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Literature summary for 1.1.3.6 extracted from

  • Praveen, V.; Srivastava, A.; Tripathi, C.K.
    Purification and characterization of the enzyme cholesterol oxidase from a new isolate of Streptomyces sp. (2011), Appl. Biochem. Biotechnol., 165, 1414-1426.
    View publication on PubMed

Application

Application Comment Organism
diagnostics the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase Streptomyces parvus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Streptomyces parvus
Ag+
-
Streptomyces parvus
DTT
-
Streptomyces parvus
Hg2+
-
Streptomyces parvus
Pb2+
-
Streptomyces parvus
Zn2+
-
Streptomyces parvus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces parvus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Streptomyces parvus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Streptomyces parvus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Streptomyces parvus
-
cholest-4-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces parvus 13647 J
-
cholest-4-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces parvus
-
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India)
-
Streptomyces parvus 13647 J
-
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India)
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 18fold by anion exchange chromatography and gel filtration Streptomyces parvus

Source Tissue

Source Tissue Comment Organism Textmining
additional information Streptomyces parvus shows markedly high cho activity in culture broth Streptomyces parvus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
purified enzyme, pH 7.2, 50°C Streptomyces parvus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta-hydroxyandrost-5-en-17-one + O2 30% of the activity with cholesterol Streptomyces parvus androst-5-en-3,17-dione + H2O2
-
?
3beta-hydroxyandrost-5-en-17-one + O2 30% of the activity with cholesterol Streptomyces parvus 13647 J androst-5-en-3,17-dione + H2O2
-
?
3beta-hydroxypregn-5-en-20-one + O2 42% of the activity with cholesterol Streptomyces parvus pregn-5-en-3,20-dione + H2O2
-
?
3beta-hydroxypregn-5-en-20-one + O2 42% of the activity with cholesterol Streptomyces parvus 13647 J pregn-5-en-3,20-dione + H2O2
-
?
5alpha-androstan-3-ol-17-one + O2 15% of the activity with cholesterol Streptomyces parvus 5alpha-androstan-3,17-dione + H2O2
-
?
5alpha-cholestan-3beta-ol + O2 equally active as cholesterol Streptomyces parvus 5alpha-cholestan-3-one + H2O2
-
?
5alpha-cholestan-3beta-ol + O2 equally active as cholesterol Streptomyces parvus 13647 J 5alpha-cholestan-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces parvus cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces parvus 13647 J cholest-4-en-3-one + H2O2
-
?
ergosta-5,7,22-trien-3beta-ol + O2 18% of the activity with cholesterol Streptomyces parvus ergostan-5,7,22-trien-3-one + H2O2
-
?
additional information no activity with cholest-5-en-3alpha-ol Streptomyces parvus ?
-
?
additional information no activity with cholest-5-en-3alpha-ol Streptomyces parvus 13647 J ?
-
?
sitost-5-en-3beta-ol + O2 88% of the activity with cholesterol Streptomyces parvus sitost-5-en-3-one + H2O2
-
?
stigmast-5-en-3beta-ol + O2 68% of the activity with cholesterol Streptomyces parvus stigmast-5-en-3-one + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Streptomyces parvus

Synonyms

Synonyms Comment Organism
CHO
-
Streptomyces parvus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Streptomyces parvus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 65 purified enzyme, stable at Streptomyces parvus
55
-
purified enzyme, 30 min, 68% activity remaining Streptomyces parvus
60
-
purified enzyme, 30 min, 66% activity remaining Streptomyces parvus
65
-
purified enzyme, 30 min, 46% activity remaining Streptomyces parvus
75
-
purified enzyme, 30 min, 14% activity remaining Streptomyces parvus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Streptomyces parvus