Information on EC 2.4.1.65 - 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.65
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RECOMMENDED NAME
GeneOntology No.
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
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GDP-beta-L-fucose + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine = GDP + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl}asparagine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Various types of N-glycan biosynthesis
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-69-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain NCTC116639
SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Syrian hamster
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
white campion
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Fucalpha(1-2)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
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?
Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Fucalpha(1-2)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
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-
-
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?
Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
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?
Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
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-
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?
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc
?
show the reaction diagram
GDP-beta-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
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-
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?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R
GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
GDP-beta-L-fucose + beta-D-Galp-(1,3)-beta-D-GlcNAcp-O-(CH2)6-NH-dansyl
GDP + beta-D-Galp-(1,3)[alpha-L-Fuc-(1,4)]-beta-D-GlcNAcp-O-(CH2)6-NH-dansyl
show the reaction diagram
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?
GDP-beta-L-fucose + Fucalpha(1->2)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + Fucalpha1,2Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc
?
show the reaction diagram
GDP-beta-L-fucose + Gal-beta-(1->3)-GlcNAc-beta-O-(CH2)8COOCH3
GDP + Gal-beta-(1->3)-[Fuc-alpha-(1->4)]-GlcNAc-beta-O-(CH2)8COOCH3
show the reaction diagram
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?
GDP-beta-L-fucose + Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + Galbeta1,3GlcNAc
?
show the reaction diagram
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3
?
show the reaction diagram
GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
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?
GDP-beta-L-fucose + NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3
?
show the reaction diagram
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?
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
?
show the reaction diagram
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the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
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?
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
show the reaction diagram
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the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
i.e. sialyl-Lex, main product of FucT-VI, i.e. VIM2, main product of FucT-V
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?
GDP-beta-L-fucose labelled with ATTO 550 + N-acetyllactosamine labelled with ATTO 647N
?
show the reaction diagram
GDP-fucose + 2-O-MeGal-beta-1,3GlcNAc
GDP + 2-O-MeGal-beta-1,3(Fuc-alpha-1,4)GlcNAc
show the reaction diagram
GDP-fucose + 2-O-MeGal-beta-1,4GlcNAc
GDP + 2-O-MeGal-beta-1,4(Fuc-alpha-1,3)GlcNAc
show the reaction diagram
100% activity
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?
GDP-fucose + Fetuin triantennary asialo agalacto glycoprotein
GDP + ?
show the reaction diagram
GDP-fucose + Fetuin triantennary asialo glycoprotein
GDP + ?
show the reaction diagram
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5
GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-O-C6H5
show the reaction diagram
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H-type 1 reaction
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?
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-sp-biotin
show the reaction diagram
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,4GlcNAc-O-C6H5
GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,3)GlcNAc-O-C6H5
show the reaction diagram
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H-type 2 reaction, hardly any activity
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?
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc-sp-biotin
show the reaction diagram
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3
GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
show the reaction diagram
GDP-fucose + Gal-beta-1,3GlcNAc
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,3GlcNAc-beta-1,3Gal-beta-1,4Glc
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-beta-1,3Gal-beta-1,4Glc
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-beta-O-(CH2)7CH3
show the reaction diagram
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type 1 reaction
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?
GDP-Fucose + Gal-beta-1,3GlcNAc-O(CH2)3NHCO(CH2)5NH-biotin
GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
show the reaction diagram
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GDP-fucose + Gal-beta-1,3GlcNAc-O-(CH2)3NHCO(CH2)5NH-biotin
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-O-(CH2)3NHCO(CH2)5NH-biotin
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,4-Glc
GDP + Gal-beta-1,4(Fuc-alpha-1,3)Glc
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3
GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
show the reaction diagram
GDP-fucose + Gal-beta-1,4GlcNAc
GDP + Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc + Gal-beta-1,4(Fuc-alpha-1,2)GlcNAc
show the reaction diagram
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enzyme activity is divided into 66% alpha-1,3FucT and 34% alpha-1,2FucT
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?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-1-R
GDP + Gal-beta-1,4(fuc-alpha-1,3)GlcNAc-beta-1-R
show the reaction diagram
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?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-O-(CH2)7CH3
GDP + Gal-beta-1,3(Fuc-alpha-1,3)GlcNAc-beta-O-(CH2)7CH3
show the reaction diagram
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type 2 reaction, hardly any activity
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?
GDP-fucose + GalNAc-beta-1,4GlcNAc-beta-O-Bn
GDP + GalNAc-beta-1,4(Fuc-alpha-1,3)GlcNAc-beta-O-Bn
show the reaction diagram
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
?
show the reaction diagram
acivity not determined
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?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
GDP + ?
show the reaction diagram
63.8% acivity
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?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
?
show the reaction diagram
acivity not determined
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?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
GDP + ?
show the reaction diagram
61.5% acivity
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?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-O-Bn
GDP + GlcNAc-beta-1,4(Fuc-alpha-1,3)GlcNAc-beta-O-Bn
show the reaction diagram
GDP-fucose + LacNAc
?
show the reaction diagram
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?
GDP-L-fucose + (Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
GDP + Fucalpha(1-3)(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
show the reaction diagram
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?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
GDP-L-fucose + 2'-fucosyllactose
GDP + ?
show the reaction diagram
GDP-L-fucose + 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn
GDP + 2-O-MeGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
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?
GDP-L-fucose + 3-O-MeGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + 3-O-MeGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
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16.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 17.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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?
GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta(1,3)Galbeta-O-Al
GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta-O-Al
show the reaction diagram
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64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 82.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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?
GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
show the reaction diagram
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64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 62.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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?
GDP-L-fucose + 3-O-sulfoGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + 3-O-sulfoGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
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10.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 14.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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?
GDP-L-fucose + 6-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
GDP + 6-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
show the reaction diagram
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3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 3.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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?
GDP-L-fucose + alpha(2,3)-sialyllactosamine
GDP + ?
show the reaction diagram
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56% of the activity with Galbeta(1,4)GlcNAc
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?
GDP-L-fucose + alpha-(2,3)-sialylated Galbeta(1,3)GlcNAc
GDP + ?
show the reaction diagram
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?
GDP-L-fucose + ancrod
GDP + ?
show the reaction diagram
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-
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?
GDP-L-fucose + asialo ancrod
GDP + ?
show the reaction diagram
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-
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?
GDP-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
GDP-L-fucose + fetuin triantennary glycopeptide
GDP + ?
show the reaction diagram
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-
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?
GDP-L-fucose + formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
GDP + ?
show the reaction diagram
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-
-
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?
GDP-L-fucose + Fuc-alpha1-2Galbeta1-4GlcNAc-octyl
GDP + ?
show the reaction diagram
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-
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?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + ?
show the reaction diagram
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-
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?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
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193% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
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?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-O-sp-biotin
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
show the reaction diagram
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118% of the activity with Galbeta3GlcNAc-O-sp-biotin
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-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-R
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)Glc
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))Glc
show the reaction diagram
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34.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 38.7% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
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-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
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3% of the activity with with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin, enzyme expressed in Sf9 cells
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?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
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9.3% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
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?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-O-sp-biotin
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O-sp-biotin
show the reaction diagram
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10.5% of the activity with Galbeta3GlcNAc-O-sp-biotin
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-
?
GDP-L-fucose + Galalpha(1,3)Galbeta(1,3)GlcNAc-R
GDP + Galalpha(1,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
GDP-L-fucose + Galalpha(1,3)Galbeta(1,3)GlcNAcbeta-O-Naph
GDP + Galalpha(1,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Naph
show the reaction diagram
-
42.6 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 46.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,3)(6-O-sulfo)GlcNAcbeta(1,3)Galbeta-O-Al
GDP + ?
show the reaction diagram
-
15.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 19.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc-O-sp-biotin
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc-R
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
-
20% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta-O-Bn
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
-
79.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 83.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)-(5-thioGlc)
GDP + ?
show the reaction diagram
-
51% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + Galbeta(1,4)Glc
GDP + Galbeta(1,4)(Fucalpha(1,3))Glc
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAc
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
2% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin, enzyme expressed in Sf9 cells
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,2)Man
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Man
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
-
33% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn
show the reaction diagram
-
1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
show the reaction diagram
-
3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 2.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbetaOallyl
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbetaOallyl
show the reaction diagram
-
64% of the activity with Galbeta(1,4)GlcNAc
-
-
-
GDP-L-fucose + Galbeta(1,4)Glucal
GDP + Galbeta(1,4)(Fucalpha(1,3))Glucal
show the reaction diagram
-
10% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + Galbeta(1,6)Galbeta(1,4)Glc
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-Me
GDP + Fucalpha(1-3)Galbeta(1-3)GlcNAcbeta-O-Me
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta1-4GlcNAc-4-nitrophenyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GalNAcbeta(1,3)(6-O-sulfo)GlcNAcbeta-O-Me
GDP + ?
show the reaction diagram
-
6.2 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 8.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + GalNAcbeta1-4GlcNAc-diaminopimelyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GalNAcbeta1-4GlcNAc-octyl
GDP + ?
show the reaction diagram
-
shows the highest catalytic activity as an acceptor substrate
-
-
?
GDP-L-fucose + IL-4 receptor
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-fucopentaose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Lewis antigen
GDP + fucosylated Lewis antigen
show the reaction diagram
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl)-acetamide
GDP + N-[(1R,3R,5R,6S,7S,8R,13R,15R,16R,17S)-6,17-dihydroxy-5-hydroxymethyl-15-methoxy-7-((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl]-acetamide
show the reaction diagram
-
low activity with FucT IV and FucT VI
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
GDP + N-[(1R,3R,5R,6S,7S,8R,13R,15R,16R,17S)-6,17-dihydroxy-5-hydroxymethyl-15-methoxy-7-((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl]-acetamide
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetyllactosamine
GDP + alpha-1,3-L-fucosyl-N-acetyllactosamine
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAc(2,3)Galbeta(1,4)Glucal
GDP + NeuAc(2,3)Galbeta(1,4)(Fucalpha(1,3))Glucal
show the reaction diagram
-
330% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAc(2,6)Galbeta(1,4)GlcNAc
GDP + NeuAc(2,6)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
-
13% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
-
57% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
-
55.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 64.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
-
620% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
show the reaction diagram
-
24.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 12.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta-O-Bn
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
show the reaction diagram
-
6.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 7.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbetaOallyl
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbetaOallyl
show the reaction diagram
-
380% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin + GDP-L-fucose
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)AspNHCO-biotin + GDP
show the reaction diagram
-
-
-
?
NeuAcalpha(2-3)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
NeuAcalpha(2-3)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
NeuAcalpha(2-3)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
pyridylamine-lacto-N-neotetraose + GDP-fucose
GDP + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R
GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
?
show the reaction diagram
-
the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
-
-
?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-1-R
GDP + Gal-beta-1,4(fuc-alpha-1,3)GlcNAc-beta-1-R
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
GDP-L-fucose + Lewis antigen
GDP + fucosylated Lewis antigen
show the reaction diagram
-
on the cell surface of hepatocarcinoma cells H7721
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Cd2+
-
stimulates
Cu2+
-
no significant activity is detected
Ni2+
-
stimulates
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-gallate
time-dependent, irreversible
2'-fucosyllactose
-
-
2,3-Dihydroxybenzoic acid
-
2,4,6-Trinitrobenzenesulfonate
-
5 mM, activity is decreased to 82%; 5 mM, activity is decreased to 86%
2,4-dihydroxybenzoic acid
-
2,5-dihydroxybenzoic acid
-
3,4,5-Trihydroxybenzoic acid
gallic acid, time-dependent, irreversible
3,4,5-trimethoxybenzoic acid
-
3,4-dihydroxybenzoic acid
-
3,4-dimethoxybenzoic acid
-
3,5-dimethoxy 4-hydroxybenzoic acid
syringic acid
4-O-beta-D-galactopyranosyl-D-glucitol
-
competitive with respect to lactose
9-(5'-O-[(alpha,beta-L-fucopyranosyl)oxycarbonylaminosulfonyl]-beta-D-ribofuranosyl)-guanine
-
poor competitive inhibition
ADP
-
about 30% inhibition at 0.4 mM
C-fucosyl analogon of GDP-fucose
-
-
-
carba-fucosyl analogon of GDP-fucose
-
-
-
Co2+
-
20 mM, 98% inhibition
Cu2+
-
20 mM, complete inhibition
Diethylpyrocarbonate
-
0.5 mM, activity is decreased to 11%; 0.5 mM, activity is decreased to 13%
ellagic acid
-
ethylenediaminetetraacetic acid
-
-
Galbeta(1,3)GalNAc
-
-
Galbeta(1,4)(3-deoxy)GlcNAcbetaOallyl
-
IC50: 710 mM
Galbeta(1,4)deoxynojirimycin
-
IC50: 8 mM
GDP-6-amino-beta-L-fucose
-
GDP-Man
-
IC50: 2 mM
GMP
-
competitive with respect to GDP-fucose
L-fucose
-
about 20% inhibition at 0.4 mM
lacto-N-fucopentaitol I
-
-
methyl gallate
-
Mn2+
-
20 mM, 40% inhibition below pH 8.0
N-bromosuccinimide
-
0.5 mM, activity is decreased to 8%; 0.5 mM, activity is decreased to 9%
propyl gallate
-
unsaturated carba-fucosyl analogon of GDP-fucose
-
-
-
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
about 70% inhibition at 0.4 mM
-
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosinediphosphate
-
about 74% inhibition at 0.4 mM
-
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
-
[1-(beta-D-lactosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phenylglyoxal
-
5 mM, FucT showed enhanced activity (114%); 5 mM, FucT shows enhanced activity (118%)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 2.7
(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
11
2'-fucosyllactose
-
-
0.16 - 0.4
2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn
0.047 - 0.1
3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
0.045
ancrod
-
enzyme form FTB
-
0.167
asialo ancrod
-
enzyme form FTB
-
0.63
fetuin triantennary asialoglycopeptide
-
enzyme form FTB
-
1.43
fetuin triantennary glycopeptide
-
enzyme form FTB
-
0.075 - 1.3
Formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
0.2 - 2.5
Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.1 - 1.3
Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
0.14
Fuc-alpha1-2Galbeta1-4GlcNAc-octyl
-
-
0.1 - 3.8
Fucalpha(1,2)Galbeta(1,3)GlcNAc
0.8
Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
1.52
Fucalpha(1,2)Galbeta(1,4)Glc
-
enzyme form FTB
0.5 - 0.7
Fucalpha(1,2)Galbeta(1,4)GlcNAc
8.4 - 22.7
Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3
0.7 - 3.3
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
0.17 - 2.2
Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3
0.6 - 12.7
Galbeta(1,3)GlcNAc
0.76 - 0.87
Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
2.4
Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
0.012
Galbeta(1,4)(5-thioGlc)
-
-
0.5
Galbeta(1,4)Glc
-
-
0.035 - 8.1
Galbeta(1,4)GlcNAc
0.4
Galbeta(1,4)GlcNAcbeta(1,2)Man
-
-
3.8
Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
0.016
Galbeta(1,4)GlcNAcbetaOallyl
-
-
0.034
Galbeta(1,4)Glucal
-
-
12
Galbeta(1,6)Galbeta(1,4)Glc
-
-
0.35 - 1.2
Galbeta(1-3)GlcNAcbeta-O-Me
0.114
Galbeta-1,3GlcNAc-R
37C, pH 6.5
5.7 - 13.3
Galbeta1,3GlcNAc-O(CH2)8CO2CH3
3.6
Galbeta1-4GlcNAc-4-nitrophenyl
-
-
3.8
GalNAcbeta1-4GlcNAc-diaminopimelyl
-
-
0.27
GalNAcbeta1-4GlcNAc-octyl
-
-
0.016
GDP
-
-
0.00573 - 0.0337
GDP-beta-L-fucose
0.1145
GDP-D-fucose
-
mutant enzyme D336A
0.03 - 0.244
GDP-fucose
0.005 - 0.035
GDP-L-fucose
0.15 - 0.154
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin
0.06
GMP
-
-
3 - 25
N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl)-acetamide
1.1 - 11
N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-Trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
0.61 - 16
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
0.4 - 5
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-Trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
0.67 - 2.5
NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn
0.1 - 0.28
NeuAcalpha(2,3)Galbeta(1,4)GlcNAc
0.77 - 3.3
NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
0.064
NeuAcalpha(2,3)Galbeta(1,4)Glucal
-
-
0.07
NeuAcalpha(2,6)Galbeta(1,4)GlcNAc
-
-
0.111
Siaalpha-2,3Galbeta-1,3GlcNAc-R
37C, pH 6.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.02 - 8.02
Fucalpha(1,2)Galbeta(1,3)GlcNAc
0.1 - 0.3
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
0.009 - 0.4
Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
8.1 - 26.12
Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3
1.1
Galbeta1,3GlcNAc-O(CH2)8CO2CH3
3.13 - 3.947
GDP-beta-L-fucose
34.33
NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3
Helicobacter pylori
Q6ST35
37C, pH 6.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007 - 0.0022
(-)-epigallocatechin-3-gallate
7
2'-fucosyllactose
-
-
0.115
2,5-dihydroxybenzoic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.00006 - 0.008
3,4,5-Trihydroxybenzoic acid
0.137
3,4-dihydroxybenzoic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
17
4-O-beta-D-galactopyranosyl-D-glucitol
-
-
0.889
C-fucosyl analogon of GDP-fucose
-
-
-
0.0671
carba-fucosyl analogon of GDP-fucose
-
-
-
0.0503
dGDP
-
at pH 4.5 and 37C
0.0012
ellagic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.0779
GDP
-
at pH 4.5 and 37C
0.102
GDP-6-amino-beta-L-fucose
-
at pH 4.5 and 37C
-
0.0807
GTP
-
at pH 4.5 and 37C
0.6
lacto-N-fucopentaitol I
-
-
0.008
methyl gallate
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.25
NEM
-
enzyme form FTA and FTB
0.045
propyl gallate
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.0256
unsaturated carba-fucosyl analogon of GDP-fucose
-
-
-
0.19
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
at pH 4.5 and 37C
-
0.186
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
-
at pH 4.5 and 37C
-
0.137
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
at pH 4.5 and 37C
-
additional information
additional information
2,3-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 2,4-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,5-dimethoxy 4-hydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4-dimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4,5-trimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20
9-(5'-O-[(alpha,beta-L-fucopyranosyl)oxycarbonylaminosulfonyl]-beta-D-ribofuranosyl)-guanine
Homo sapiens
-
-
0.049
dGDP
Homo sapiens
-
at pH 4.5 and 37C
710
Galbeta(1,4)(3-deoxy)GlcNAcbetaOallyl
Homo sapiens
-
IC50: 710 mM
8
Galbeta(1,4)deoxynojirimycin
Homo sapiens
-
IC50: 8 mM
0.059
GDP
Homo sapiens
-
at pH 4.5 and 37C
0.104
GDP-6-amino-beta-L-fucose
Homo sapiens
-
at pH 4.5 and 37C
-
2
GDP-Man
Homo sapiens
-
IC50: 2 mM
0.094
GTP
Homo sapiens
-
at pH 4.5 and 37C
0.134
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37C
-
0.247
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37C
-
0.139
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000001
-
old leaves; sepal
0.000002
-
old roots; petal; shoot
0.000015
-
pistill; root
0.000026
-
young leaves
0.000039
-
young roots
0.000045
-
H-type 1 acceptor (Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5)
0.000052
-
type 1 acceptor (Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3)
0.000265
-
H-type 1 acceptor (Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5)
0.000312
-
type 1 acceptor (Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3)
0.0163
alpha 1,3 activity, wild type, strain NCTC116639
0.0238
alpha-1,3 activity, alpha-1,4 activity is one third of the alpha 1,3-activity
0.061
-
-
1.13
-
reaction with lacto-N-fucopentaose I
2.05
-
reaction with 2'-fucosyllactose
72
-
enzyme expressed in Trichoplusia ni
567
-
enzyme expressed in Sf9 cells
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
in the absence of Mn2+
6
-
assay at
6.5
-
assay at
7 - 7.8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
in the presence of Mn2+
6.2 - 8.5
-
pH 6.2: about 70% of maximal activity, pH 8.5: about 90% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
-
soluble form, isoelectric gel electrophoresis, chromatofocusing
8.9
-
membrane enzyme, isoelectric gel electrophoresis, chromatofocusing
additional information
-
above 10.0, membrane enzyme, isoelectric gel electrophoresis, chromatofocussing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
; low expression of FUT1-6 and FUT9, highest expression of FUT10, FUT11 is absent
Manually annotated by BRENDA team
FUT10-391 and FUT10-419
Manually annotated by BRENDA team
-
cystic fibrosis airway epithelial cell
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
-
hepatocarcinoma cell, expression levels of alpha1,3FucT enzyme types in descending order: alphaFucT IV, III, VI, VI, VII, and IX
Manually annotated by BRENDA team
adult tissue with weak level
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
-
; very low expression of FUT1, low expression of FUT2, higher expression of FUT4-6, highest expression of FUT3 and FUT10-11
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
; very low expression of FUT1, FUT5 and FUT6, low expression of FUT2, higher expression of FUT9-11, FUT3 is absent, highest expression of FUT4
Manually annotated by BRENDA team
fetal tissue with weak level
Manually annotated by BRENDA team
-
FT3, FT6, and FT7
Manually annotated by BRENDA team
adult tissue with weak level
Manually annotated by BRENDA team
-
FT4 and FT9
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
fetal tissue, intensity similar to in the embryos
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
adult tissue with moderate level
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
fusion proteins PIR1-HA-FUT6 and PIR2-FLAG-FUT6
Manually annotated by BRENDA team
FUT10-419 and FUT10-479, FUT10-479 has the same trans-membrane domain and NH2-terminal endoplasmic reticulum membrane retention signal found in the FUT10-419 splice variant plus a new endoplasmic reticulum membrane retention signal in the COOH terminus; FUT11-476 has no endoplasmic reticulum membrane retention signal, but the FUT11-492 variant has a KKXX-like motif in its COOH terminus that can retain it in the endoplasmic reticulum
Manually annotated by BRENDA team
-
FT3dc mutant
-
Manually annotated by BRENDA team
-
FT3 wildtype
-
Manually annotated by BRENDA team
-
Golgi type II membrane protein
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41800
-
SDS-PAGE, immunoblot
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 41859, calculation from nucleotide sequence
additional information
-
the enzyme is present in an equilibrium of monomer/dimer in the trans-Golgi/trans-Golgi-network of transfected BHK cells
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
additional information
-
the enzyme expressed in Tn cell line has a lower global charge, possibly due to post-translational modifications, such as phosphorylatopm or sulfation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
stable up to 45C; the enzyme is stable up to 45C
50
-
stable up to
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50% glycerol, protein concentration 0.03 mg/ml
-
-70C, recombinant enzyme is stable for several months in cacodylate buffer containing
-80C, 3 months, lyophilized UA948FucT(1-428) loses 23% of its activity
-
-80C, several months
-
most stable at -80C with or without glycerol supplementation
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anion exchange-chromatography on a CM-Sepharose column, followed by an affinity chromatography on a GDP Fractogel column
-
Mono Q column chromatography and Sephadex G-25 gel filtration
-
native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chromatography; native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chromatography
secretory variant of enzyme
-
two molecular forms: FTA and FTB
-
UA948FucT(1-428) with a His6 tag at the C terminus is purified
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of chimeric FucTs, expression in Escherichia coli HMS174DE3 cells
construction of plasmids encoding soluble forms of the recombinant human FucT-III where the human IL-2 sequence is linked to Ala47 or Val-36 of the FucT-III and expression in stable transfected BHK-21 cell lines
-
expression in BHK-21B cells
-
expression in COS cells
-
expression in Escherichia coli
expression in HEK 293 cells
expression in Saccharomyces cerevisiae, construction of fusion genes PIR1-HA-FUT6 and PIR2-FLAG-FUT6
-
expression in Spodoptera frugiperda (Sf9) insect cells
-
expression in Spodoptera frugiperda SF-9 cells, the secreted activity SFT3 increases until the 6th day of culture when it reaches the value 1.9 mU x 10e-6 cells and 13.4 mg/l, whereas only 5% of activity is retained inside the cells
-
expression of a secreted form of Fuc-TIII, SFT3, in two insect cell lines, Spodoptera frugiperda and Trichoplusia ni using the baculovirus expression system. The enzyme from the Tn cell line has a lower global charge, possibly due to post-translational modifications, such as phosphorylation or sulfation
-
expression of alpha-1,3/4-fucosyltransferase in the gastric pit cell lineage of FVB/N transgenic mice
-
expression of wild-type and mutant enzyme in COS-7 cells
-
full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3
-
plasmid ProtA-FucT-VI, encoding a soluble chimaeric protein consisting of a part of protein A fused to the catalytic domain of FucT-VI, transfected into Cos-7 cells
-
stable expression of FucT IV and IV in CHO cells
-
transient expression of cDNA splice variants FUT10-391, FUT10-419, and FUT10-479, and GFP-tagged-FUT10 cDNA constructs from vector PCR3.1 in COS-7 Cells; transient expression of cDNA splice variants FUT11-476 and FUT11-492 from vector PCR3.1 in COS-7 Cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
decrease in FUT4 transcript levels in MKN45 confluent cells
-
FT3, FT6, and FT7 are markedly elevated in bone- and liver-metastatic prostate cancer and dictate synthesis of sialyl Lewis X and endothelial selectin ligands on metastatic prostate cancer cells
-
FT4 expression is 3- to 15fold lower in metastatic specimens
-
increase in mRNA levels of FUT5 and FUT6 in MKN45 confluent cells
-
the enzyme is upregulated 3fold in hepatocellular carcinoma cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D112E
-
muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors
D125E
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
D336A
-
fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V
E111D
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
I126V
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
K73T
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/E111D
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/I75V/T87A
-
insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme
K73T/R110W
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K97S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
R110H
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110K
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110N
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
R96S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
S110R
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
S111K
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
T87K
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/D125E
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/V89I/A101T
-
insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme
T87K/W124R
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
V112I
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
W111A
-
alpha-1,4-activity is decreased to 10-20% of the wild type activity
W111F
-
alpha-1,4-activity is decreased to 42% of the wild type activity
W111R
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors
W111Y
-
alpha-1,4-activity is decreased to 58% of the wild type activity
W11R/D112E
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors. Increased type 2 activity compared to mutant W111R
W124A
-
alpha-1,4-activity is decreased to 20% of the wild type activity
W124F
-
alpha-1,4-activity is decreased to 17% of the wild type activity
W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
W124V
-
undetectable alpha-1,4-activity
W124Y
-
alpha-1,4-activity is decreased to 52% of the wild type activity
FT3dc
-
mutant where the cytoplasmic domain (Asp-2 to Trp-13) is deleted
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
-
the stable system using the expression vector pIB/Vf-His-TOPO constitutes an advance for the large scale expression of glycosyltransferases and possibly other glycoproteins in insect cells
synthesis