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Literature summary for 2.4.1.65 extracted from

  • Mollicone, R.; Moore, S.E.; Bovin, N.; Garcia-Rosasco, M.; Candelier, J.J.; Martinez-Duncker, I.; Oriol, R.
    Activity, splice variants, conserved peptide motifs, and phylogeny of two new alpha1,3-fucosyltransferase families (FUT10 and FUT11) (2009), J. Biol. Chem., 284, 4723-4738.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of cDNA splice variants FUT10-391, FUT10-419, and FUT10-479, and GFP-tagged-FUT10 cDNA constructs from vector PCR3.1 in COS-7 Cells Homo sapiens
transient expression of cDNA splice variants FUT11-476 and FUT11-492 from vector PCR3.1 in COS-7 Cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
GDP-L-fucose FUT10-391 Homo sapiens
0.015
-
GDP-L-fucose FUT10-479 Homo sapiens
0.15
-
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin FUT10-479 Homo sapiens
0.154
-
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin FUT10-391 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum FUT10-419 and FUT10-479, FUT10-479 has the same trans-membrane domain and NH2-terminal endoplasmic reticulum membrane retention signal found in the FUT10-419 splice variant plus a new endoplasmic reticulum membrane retention signal in the COOH terminus Homo sapiens 5783
-
endoplasmic reticulum FUT11-476 has no endoplasmic reticulum membrane retention signal, but the FUT11-492 variant has a KKXX-like motif in its COOH terminus that can retain it in the endoplasmic reticulum Homo sapiens 5783
-
Golgi apparatus FUT10-391 is a soluble protein without a transmembrane domain or endoplasmic reticulum retention signal that transiently localizes to the Golgi and then is routed to the lysosome Homo sapiens 5794
-
membrane FUT10-419 and FUT10-479 have a type II trans-membrane topology. FUT10-479 has, in addition, a COOH-endoplasmic reticulum membrane retention signal Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information FUT10 functions well without MnCl2 Homo sapiens
additional information FUT11 functions well without MnCl2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q495W5
-
-
Homo sapiens Q6P4F1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain FUT10-479, adult tissue with low level Homo sapiens
-
colon adult tissue with high level Homo sapiens
-
embryo FUT10-391 and FUT10-419 Homo sapiens
-
gall bladder adult tissue with high level Homo sapiens
-
ileum adult tissue with weak level Homo sapiens
-
jejunum adult tissue with high level Homo sapiens
-
kidney fetal tissue with weak level, adult tissue with moderate level Homo sapiens
-
liver fetal tissue, intensity similar to in the embryos Homo sapiens
-
lung fetal and adult tissue with weak level Homo sapiens
-
additional information adult spleen, heart, muscle, liver, and pancreas do not express any of the FUT10 transcripts Homo sapiens
-
muscle fetal tissue with weak level Homo sapiens
-
placenta adult tissue with weak level Homo sapiens
-
rectum adult tissue with high level Homo sapiens
-
skin fetal tissue, intensity similar to in the embryos Homo sapiens
-
small intestine fetal tissue, intensity similar to in the embryos Homo sapiens
-
stomach adult tissue with high level Homo sapiens
-
uterus adult tissue with moderate level Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose FUT10 activity toward the acceptor 0989-BM is increased by about 50% compared with those observed using BGA-biotin Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin + GDP-L-fucose
-
Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)AspNHCO-biotin + GDP
-
?

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase
-
Homo sapiens
FUT10
-
Homo sapiens
FUT11
-
Homo sapiens