Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzyme in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D125E | site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
E111D | site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
I126V | site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
K73T | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
K73T/E111D | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
K73T/I75V/T87A | insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
K73T/R110W | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
K73T/R110W/E111D/V112I | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
K97S | site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
additional information | construction of several chimeric mutants by domain swappig, overview | Homo sapiens |
R110W | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
R110W/E111D/V112I | site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
R96S | site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
S110R | site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
S111K | site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
T87K | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
T87K/D125E | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
T87K/V89I/A101T | insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
T87K/W124R | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
T87K/W124R/D125E/I126V | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
V112I | site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity | Homo sapiens |
W124R | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
W124R/D125E/I126V | site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | Homo sapiens | the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activities of wild-type and mutant enzymes, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex | Homo sapiens | ? | - |
? | |
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex | Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | i.e. sialyl-Lex, main product of FucT-VI | ? | |
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex | Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide | i.e. VIM2, main product of FucT-V | ? | |
additional information | FucT-III and FucT-V catalyze fucose transfer to Galbeta1-3GlcNAc chain acceptors in an alpha1,4-linkage, these two enzymes have distinct site-specific fucosylation properties, site-specific fucosylation is defined within a 40-amino acid segment containing 8 amino acid differences between the two enzymes, FucT-VI has about 12fold higher activity compared with FucT-V with the Trp124/Arg110 site in the enzymes being responsible primarily for this activity difference, substrate specificities of wild-type and mutant enzymes, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha1,3/4-fucosyltransferases-V and -VI | - |
Homo sapiens |
FucT-V | - |
Homo sapiens |
FucT-VI | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |