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Literature summary for 2.4.1.65 extracted from

  • Prieels, J.P.; Monnom, D.; Dolmans, M.; Beyer, T.A.; Hill, R.L.
    Co-purification of the Lewis blood group N-acetylglucosaminide alpha 1-4 fucosyltransferase and an N-acetylglucosaminide alpha 1-3 fucosyltransferase from human milk (1981), J. Biol. Chem., 256, 10456-10463.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2'-fucosyllactose
-
Homo sapiens
4-O-beta-D-galactopyranosyl-D-glucitol competitive with respect to lactose Homo sapiens
GDP competitive with respect to GDP-fucose Homo sapiens
GMP competitive with respect to GDP-fucose Homo sapiens
lacto-N-fucopentaitol I
-
Homo sapiens
NEM 3 mM, 59°C Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
GDP-L-fucose reaction with Galbeta(1,4)GlcNAc Homo sapiens
0.0105
-
GDP-L-fucose reaction with Galbeta(1,3)GlcNAc Homo sapiens
0.0131
-
GDP-L-fucose reaction with lactose Homo sapiens
0.016
-
GDP
-
Homo sapiens
0.06
-
GMP
-
Homo sapiens
1.6
-
Galbeta(1,4)GlcNAc
-
Homo sapiens
1.9
-
Galbeta(1,3)GlcNAc
-
Homo sapiens
2.4
-
Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
Homo sapiens
3.8
-
Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
Homo sapiens
11
-
2'-fucosyllactose
-
Homo sapiens
12
-
Galbeta(1,6)Galbeta(1,4)Glc
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ stimulates Homo sapiens
Ca2+ stimulates Homo sapiens
Cd2+ stimulates Homo sapiens
Co2+ stimulates Homo sapiens
Mg2+ stimulates, 20 mM required for maximal activation Homo sapiens
Mn2+ stimulates, activation is maximal at 5 mM Homo sapiens
Ni2+ stimulates Homo sapiens
Zn2+ stimulates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
x * 51000 + x * 53000, SDS-PAGE Homo sapiens
53000
-
x * 51000 + x * 53000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13
-
reaction with lacto-N-fucopentaose I Homo sapiens
2.05
-
reaction with 2'-fucosyllactose Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, protein concentration 0.03 mg/ml Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + 2'-fucosyllactose 11% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + ?
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc i.e. lacto-N-fucopentaose I Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc 29% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc 20% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
?
GDP-L-fucose + Galbeta(1,4)Glc i.e. lactose, 2% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))Glc
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAc 43% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,2)Man 28% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Man
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc 33% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
?
GDP-L-fucose + Galbeta(1,6)Galbeta(1,4)Glc
-
Homo sapiens GDP + ?
-
?

Subunits

Subunits Comment Organism
? x * 51000 + x * 53000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.8
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.5 pH 6.2: about 70% of maximal activity, pH 8.5: about 90% of maximal activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
lacto-N-fucopentaitol I
-
Homo sapiens
7
-
2'-fucosyllactose
-
Homo sapiens
17
-
4-O-beta-D-galactopyranosyl-D-glucitol
-
Homo sapiens