Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-fucosyllactose | - |
Homo sapiens | |
4-O-beta-D-galactopyranosyl-D-glucitol | competitive with respect to lactose | Homo sapiens | |
GDP | competitive with respect to GDP-fucose | Homo sapiens | |
GMP | competitive with respect to GDP-fucose | Homo sapiens | |
lacto-N-fucopentaitol I | - |
Homo sapiens | |
NEM | 3 mM, 59°C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
GDP-L-fucose | reaction with Galbeta(1,4)GlcNAc | Homo sapiens | |
0.0105 | - |
GDP-L-fucose | reaction with Galbeta(1,3)GlcNAc | Homo sapiens | |
0.0131 | - |
GDP-L-fucose | reaction with lactose | Homo sapiens | |
0.016 | - |
GDP | - |
Homo sapiens | |
0.06 | - |
GMP | - |
Homo sapiens | |
1.6 | - |
Galbeta(1,4)GlcNAc | - |
Homo sapiens | |
1.9 | - |
Galbeta(1,3)GlcNAc | - |
Homo sapiens | |
2.4 | - |
Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
Homo sapiens | |
3.8 | - |
Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
Homo sapiens | |
11 | - |
2'-fucosyllactose | - |
Homo sapiens | |
12 | - |
Galbeta(1,6)Galbeta(1,4)Glc | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | stimulates | Homo sapiens | |
Ca2+ | stimulates | Homo sapiens | |
Cd2+ | stimulates | Homo sapiens | |
Co2+ | stimulates | Homo sapiens | |
Mg2+ | stimulates, 20 mM required for maximal activation | Homo sapiens | |
Mn2+ | stimulates, activation is maximal at 5 mM | Homo sapiens | |
Ni2+ | stimulates | Homo sapiens | |
Zn2+ | stimulates | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000 + x * 53000, SDS-PAGE | Homo sapiens |
53000 | - |
x * 51000 + x * 53000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.13 | - |
reaction with lacto-N-fucopentaose I | Homo sapiens |
2.05 | - |
reaction with 2'-fucosyllactose | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, protein concentration 0.03 mg/ml | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + 2'-fucosyllactose | 11% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + ? | - |
? | |
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | i.e. lacto-N-fucopentaose I | Homo sapiens | GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
? | |
GDP-L-fucose + Galbeta(1,3)GlcNAc | 29% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc | - |
? | |
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | 20% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
? | |
GDP-L-fucose + Galbeta(1,4)Glc | i.e. lactose, 2% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))Glc | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAc | 43% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,2)Man | 28% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Man | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc | 33% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc | Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
? | |
GDP-L-fucose + Galbeta(1,6)Galbeta(1,4)Glc | - |
Homo sapiens | GDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000 + x * 53000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.8 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.5 | pH 6.2: about 70% of maximal activity, pH 8.5: about 90% of maximal activity | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
lacto-N-fucopentaitol I | - |
Homo sapiens | |
7 | - |
2'-fucosyllactose | - |
Homo sapiens | |
17 | - |
4-O-beta-D-galactopyranosyl-D-glucitol | - |
Homo sapiens |