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Literature summary for 2.4.1.65 extracted from

  • Leonard, R.; Lhernould, S.; Carlue, M.; Fleurat, P.; Maftah, A.; Costa, G.
    Biochemical characterization of Silene alba alpha4-fucosyltransferase and Lewis a products (2005), Glycoconj. J., 22, 71-78.
    View publication on PubMed
Search for more literature for 2.4.1.65

Activating Compound

Activating Compound Comment Organism Structure
Phenylglyoxal 5 mM, FucT showed enhanced activity (114%) Silene latifolia subsp. alba
Phenylglyoxal 5 mM, FucT shows enhanced activity (118%) Silene latifolia subsp. alba

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonate 5 mM, activity is decreased to 82%; 5 mM, activity is decreased to 86% Silene latifolia subsp. alba
diethylpyrocarbonate 0.5 mM, activity is decreased to 11%; 0.5 mM, activity is decreased to 13% Silene latifolia subsp. alba
ethylenediaminetetraacetic acid
-
 Silene latifolia subsp. alba
additional information the Silene alba alpha-4-FucT is insensitive to N-ethylmaleimide treatment (5 mM), in contrast to human FUT3 Silene latifolia subsp. alba
N-bromosuccinimide 0.5 mM, activity is decreased to 8%; 0.5 mM, activity is decreased to 9% Silene latifolia subsp. alba
Zn2+
-
 Silene latifolia subsp. alba

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Silene latifolia subsp. alba 139
-
soluble
-
 Silene latifolia subsp. alba
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Silene latifolia subsp. alba
Mn2+ required for activity Silene latifolia subsp. alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 above 100 kDa for the membrane-anchored enzyme, selective ultra-filtration Silene latifolia subsp. alba
additional information
-
 between 50 and 100 kDa for the soluble enzyme, selective ultra-filtration Silene latifolia subsp. alba

Organism

Organism UniProt Comment Textmining
Silene latifolia subsp. alba
-
 white campion
-

Purification (Commentary)

Purification (Comment) Organism
-
 Silene latifolia subsp. alba

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Silene latifolia subsp. alba
-
petal
-
 Silene latifolia subsp. alba
-
pistil
-
 Silene latifolia subsp. alba
-
root
-
 Silene latifolia subsp. alba
-
sepal
-
 Silene latifolia subsp. alba
-
shoot
-
 Silene latifolia subsp. alba
-
stamen
-
 Silene latifolia subsp. alba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 below 0.0000001, H-type 2 acceptor (Fuc-alpha-1,2Gal-beta-1,4GlcNAc-O-C6H5) Silene latifolia subsp. alba
additional information
-
 below 0.0000001, type 2 acceptor (Gal-beta-1,4GlcNAc-beta-O-(CH2)7CH3) Silene latifolia subsp. alba
0.000001
-
 old leaves Silene latifolia subsp. alba
0.000001
-
 sepal Silene latifolia subsp. alba
0.000002
-
 shoot Silene latifolia subsp. alba
0.000002
-
 old roots Silene latifolia subsp. alba
0.000002
-
 petal Silene latifolia subsp. alba
0.000015
-
 root Silene latifolia subsp. alba
0.000015
-
 pistill Silene latifolia subsp. alba
0.000026
-
 young leaves Silene latifolia subsp. alba
0.000039
-
 young roots Silene latifolia subsp. alba
0.000045
-
 H-type 1 acceptor (Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5) Silene latifolia subsp. alba
0.00005
-
 seedling Silene latifolia subsp. alba
0.000052
-
 type 1 acceptor (Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3) Silene latifolia subsp. alba
0.000067
-
 stamen Silene latifolia subsp. alba
0.000265
-
 H-type 1 acceptor (Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5) Silene latifolia subsp. alba
0.000312
-
 type 1 acceptor (Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3) Silene latifolia subsp. alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,3GlcNAc-O-C6H5 H-type 1 reaction Silene latifolia subsp. alba GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-O-C6H5
-
 ?
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,4GlcNAc-O-C6H5 H-type 2 reaction, hardly any activity Silene latifolia subsp. alba GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,3)GlcNAc-O-C6H5
-
 ?
GDP-fucose + Gal-beta-1,3GlcNAc-beta-O-(CH2)7CH3 type 1 reaction Silene latifolia subsp. alba GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-beta-O-(CH2)7CH3
-
 ?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-O-(CH2)7CH3 type 2 reaction, hardly any activity Silene latifolia subsp. alba GDP + Gal-beta-1,3(Fuc-alpha-1,3)GlcNAc-beta-O-(CH2)7CH3
-
 ?

Synonyms

Synonyms Comment Organism
alpha-4-fucosyltransferase
-
 Silene latifolia subsp. alba
FucT
-
 Silene latifolia subsp. alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Silene latifolia subsp. alba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
 Silene latifolia subsp. alba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to 45°C Silene latifolia subsp. alba
45
-
 the enzyme is stable up to 45°C Silene latifolia subsp. alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Silene latifolia subsp. alba

pI Value

Organism Comment pI Value Maximum pI Value
Silene latifolia subsp. alba above 10.0, membrane enzyme, isoelectric gel electrophoresis, chromatofocussing
-
 additional information
Silene latifolia subsp. alba soluble form, isoelectric gel electrophoresis, chromatofocusing
-
 8.8
Silene latifolia subsp. alba membrane enzyme, isoelectric gel electrophoresis, chromatofocusing
-
 8.9