2.7.7.31: DNA nucleotidylexotransferase
This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.
Word Map on EC 2.7.7.31
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2.7.7.31
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dutp
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nick
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tunel
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nick-end
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caspase-3
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leukemia
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tdt-mediated
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necrosis
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bcl-2
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marrow
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tunel-positive
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lymphoblast
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lymphocyte
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neuroprotective
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sham
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lymphoid
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ischemia
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eosin
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infarct
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b-cell
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hematoxylin
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end-labeling
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deoxyuridine
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cerebral
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thymus
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polymerases
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malondialdehyde
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reperfusion
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thymocytes
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ladder
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cardiomyocytes
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anti-apoptotic
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ischemia-reperfusion
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immunophenotype
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myeloid
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immunoglobulin
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dutp-biotin
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occlusion
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nissl
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labeling-positive
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analysis
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french-american-british
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3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
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blastic
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internucleosomal
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t-lymphoblastic
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apoptosis-related
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transcriptases
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2'-deoxyuridine
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biotechnology
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klenow
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calla
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medicine
- 2.7.7.31
- dutp
- nick
-
tunel
-
nick-end
- caspase-3
- leukemia
-
tdt-mediated
- necrosis
- bcl-2
- marrow
-
tunel-positive
- lymphoblast
- lymphocyte
-
neuroprotective
-
sham
-
lymphoid
- ischemia
- eosin
- infarct
- b-cell
- hematoxylin
-
end-labeling
- deoxyuridine
- cerebral
- thymus
- polymerases
- malondialdehyde
-
reperfusion
- thymocytes
-
ladder
-
cardiomyocytes
-
anti-apoptotic
-
ischemia-reperfusion
-
immunophenotype
- myeloid
- immunoglobulin
-
dutp-biotin
- occlusion
-
nissl
-
labeling-positive
- analysis
-
french-american-british
-
3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
-
blastic
-
internucleosomal
-
t-lymphoblastic
-
apoptosis-related
- transcriptases
- 2'-deoxyuridine
- biotechnology
-
klenow
- calla
- medicine
Reaction
Synonyms
addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase
ECTree
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Application
Application on EC 2.7.7.31 - DNA nucleotidylexotransferase
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analysis
biotechnology
medicine
DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates. Amino- and nitrophenyl-modified dNTPs are good substrates giving 3'-end stretches of different lengths depending on the nucleotide and concentration. d[7-Deaza-7-(3-nitrophenyl)]GTP is efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide, resulting in a considerable enhancement of voltammetric signals due to the nitro group reduction. Discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes is easily accomplished, and tumour suppressor p53 protein is able to recognize a specific binding site within tail-labelled DNA substrates
analysis
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reliable and accurate 3'-end miRNA-labeling method for microarray detection by terminal-deoxynucleotidyl transferase TdT. Using its ability to add polynucleotides at a RNA receptor molecule by using deoxycytidine triphosphate, miRNA is successfully labeled by adding fluorescence deoxycytidine triphosphates to its 3'-end. The TdT-labeling method can detect as little as 0.04 fmol of synthetic small RNA, and produce precise and accurate measurements that span a linear dynamic range from 0.04 to 5 fmol of synthetic small RNA
analysis
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construction of a nonhomologous end-joining assay vector NAV, containing mKate2, Venus and ccdB genes. Cotransfection of NAV with a construct expressing the restriction enzyme I-SceI generates a double-strand break in NAV that excises mKate2 and ccdB. Repair of this double-strand break produces an intact vector that expresses Venus, a green fluorescent protein. Cells bearing the repaired NAV lack the ccdB gene which slows cell proliferation, the cultures are enriched in cells containing repaired double-strand breaks
analysis
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methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase TdT using a thioflavin T probe. The method is highly selective and sensitive. The fluorescence intensity is directly proportional to Dam MTase concentration in the range from 0.1 to 8.0 U/ml with a detection limit of 0.1 U/ml. As no labeling with a fluorophore quencher pair is required, the method is simple and low cost
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use in production of synthetic homo- and heteropolymers, N-acetylation or N-alkylation of derivatives of dNTPs
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little reliability of enzyme as marker of lymphoblastic lymphoma and leukemia