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2.7.7.31: DNA nucleotidylexotransferase

This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.

Word Map on EC 2.7.7.31

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.31 DNA nucleotidylexotransferase

Inhibitors

Inhibitors on EC 2.7.7.31 - DNA nucleotidylexotransferase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2S)-1-(6-aminopurin-9-yl)-2-(Hydroxymethyl)cyclopentane
-
-
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate
-
-
1,10-phenanthroline
-
not m-isomer
2',3'-dideoxyadenosine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate ATP, 50% inhibition at 0.006 mM
2',3'-dideoxycytidine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate CTP, 50% inhibition at 0.002 mM
2',3'-dideoxyguanosine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate GTP, 50% inhibition at 0.005 mM
2',3'-dideoxythymidine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate TTP, 50% inhibition at 0.020 mM
2',3'-dideoxythymidine 5'-triphosphate
-
-
2,2'-dipyridyl
-
-
3'-azido-TTP
-
-
3'-dATP
-
selective inhibitor, inactive in DNA-synthesizing systems both with DNA polymerase alpha or beta
3'-Deoxy-3'-fluorothymidine 5'-triphosphate
-
-
5-(hydroxymethyl)-2-furfural
-
HMF, a pyrolysate of carbohydrate isolated from instant coffee (CoVea arabica L.)
6-(1-phenyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
-
IC50: 0.0472 mM
6-(1-phenylmethyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
-
IC50: 0.0079 mM
6-(4-benzoyl-1-phenylmethyl-1H-pyrrol-2-yl)-2,4-dioxo-5-hexenoic acid
-
IC50: 0.0162 mM
9-alpha-Arabinofuranosyladenine 5'-triphosphate
-
-
9-beta-arabinofuranosyladenine 5'-triphosphate
-
-
Ap5A
-
-
aurintricarboxylic acid
-
-
Baicalin
-
competitive versus the DNA primer and non-competitive versus the dNTP substrate, IC50: 0.0186 mM
cordycepin
-
selectively cytotoxic against TdT+ leukemic cells, particularly in combination with the adenosine deaminase inhibitor deoxycoformycin
cysteine
-
-
D-/L-carbocyclic dideoxynucleoside triphosphates
-
-
-
dATP
-
mechanism of Mn2+-dependent inhibition
ddATP
-
-
ddCTP
-
-
ddGTP
-
-
ddTTP
-
-
diadenosine 5',5'-diphosphate
-
-
diadenosine 5',5'-hexaphosphate
-
-
diadenosine 5',5'-pentaphosphate
-
-
diadenosine 5',5'-tetraphosphate
diadenosine 5',5'-triphosphate
-
-
diallyl trisulfide
-
-
diphosphate
EDTA
-
-
genistein
-
competitive versus the DNA primer and non-competitive versus the dNTP substrate, IC50: 0.0287 mM
high ionic strength
-
-
-
histidine
-
-
L-beta-deoxythymidine-triphosphate
-
i.e. L-dTTP, competitive
L-dTTP
-
-
Mercaptoacetate
-
-
metal chelators
-
-
-
N-ethylmaleimide
-
-
NH4+
-
-
Oligo(dA)
-
product inhibition
P1,P5-diadenosine 5'-pentaphosphate
-
-
phosphate
-
-
pyran
-
-
Streptolydigin
-
does not significantly inhibit DNA polymerase alpha, beta, and gamma, or RNA polymerase, non-competitive to (dA)12-18 and dGTP
TdT interacting factor 1
-
negatively regulates TdT activity, TdT interacting factor 1 binds to the Pol beta-like region in TdT and blocks TdT access to DNA ends
-
triethylenetetramine
-
-
additional information
-
Allium sativa extract is a mixture of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide exhibiting an IC50 value of 0.371 mM at 37°C and a KI value of 0.0121 mM at 37°C
-