Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.7.31: DNA nucleotidylexotransferase

This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.

Word Map on EC 2.7.7.31

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.31 DNA nucleotidylexotransferase

Metals Ions

Metals Ions on EC 2.7.7.31 - DNA nucleotidylexotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
the enzyme can utilize several different metal ions including Mg2+, Co2+, Mn2+ and Zn2+, though its preference for nucleotide incorporation depends on the metal ion used. For example, when the buffer is supplemented with Mg2+, the purine nucleotide incorporation rate is 10fold higher than that of pyrimidines. When the buffer is supplemented with Co2+, the pyrimidine nucleotide incorporation rate is higher. Supplementing Mg2+ with sub-millimolar concentrations of Zn2+ increases the enzyme activity 2-4fold