2.7.7.31: DNA nucleotidylexotransferase
This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.
Word Map on EC 2.7.7.31
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2.7.7.31
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dutp
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nick
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tunel
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nick-end
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caspase-3
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leukemia
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tdt-mediated
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necrosis
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bcl-2
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marrow
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tunel-positive
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lymphoblast
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lymphocyte
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neuroprotective
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sham
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lymphoid
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ischemia
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eosin
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infarct
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b-cell
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hematoxylin
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end-labeling
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deoxyuridine
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cerebral
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thymus
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polymerases
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malondialdehyde
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reperfusion
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thymocytes
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ladder
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cardiomyocytes
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anti-apoptotic
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ischemia-reperfusion
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immunophenotype
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myeloid
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immunoglobulin
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dutp-biotin
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occlusion
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nissl
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labeling-positive
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analysis
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french-american-british
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3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
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blastic
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internucleosomal
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t-lymphoblastic
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apoptosis-related
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transcriptases
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2'-deoxyuridine
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biotechnology
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klenow
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calla
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medicine
- 2.7.7.31
- dutp
- nick
-
tunel
-
nick-end
- caspase-3
- leukemia
-
tdt-mediated
- necrosis
- bcl-2
- marrow
-
tunel-positive
- lymphoblast
- lymphocyte
-
neuroprotective
-
sham
-
lymphoid
- ischemia
- eosin
- infarct
- b-cell
- hematoxylin
-
end-labeling
- deoxyuridine
- cerebral
- thymus
- polymerases
- malondialdehyde
-
reperfusion
- thymocytes
-
ladder
-
cardiomyocytes
-
anti-apoptotic
-
ischemia-reperfusion
-
immunophenotype
- myeloid
- immunoglobulin
-
dutp-biotin
- occlusion
-
nissl
-
labeling-positive
- analysis
-
french-american-british
-
3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
-
blastic
-
internucleosomal
-
t-lymphoblastic
-
apoptosis-related
- transcriptases
- 2'-deoxyuridine
- biotechnology
-
klenow
- calla
- medicine
Reaction
Synonyms
addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase
ECTree
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General Information
General Information on EC 2.7.7.31 - DNA nucleotidylexotransferase
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metabolism
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enzyme is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. Enzyme is ubiquitylated by the Cul3-based ubiquitylation system in vitro and may also be directly ubiquitylated by the E2 proteins UbcH5a/b/c and UbcH6, E3-independently. Ubiquitylation inhibits the nucleotidyltransferase activity
physiological function
in Chinese hamster ovary cells, ectopic expression of the enzmye efficiently promotes N-additions at the junction of chromosomal double-strand breaks generated by the meganuclease I-SceI. The size of the N-additions is comparable with that at V(D)J junctions. No N-addition is observed in KU80- or XRCC4-deficient cells
physiological function
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in cells overexpressing terminal deoxynucleotidyltransferase, the enzyme suppresses DNA repair that is based on short (one- or two-base) homology regions, to efficiently add deoxynucleotides during VDJ recombination in lymphoid cells
physiological function
TdT also has a templated-polymerase activity, in the presence of higher concentrations of a downstream DNA duplex, and performs a micro-homology single base-pair search to align the DNA synapsis. Both templated and untemplated activities of TdT are needed to explain the distributions of lengths of N regions observed experimentally in T cell receptors and antibodies
physiological function
the genetic variations generated by the hTdT single-nucleotide polymorphisms will vary the human immune repertoire and thus its responses. In vitro, six natural variants of TdT differ from wild-type TdT in polymerization ability, with four having significantly lower activity. In vivo, the presence of TdT varies both the efficiency of recombination and N-addition, with two variants generating coding joints with significantly fewer N-additions