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2.7.7.31: DNA nucleotidylexotransferase

This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.

Word Map on EC 2.7.7.31

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.31 DNA nucleotidylexotransferase

Crystallization

Crystallization on EC 2.7.7.31 - DNA nucleotidylexotransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
high-resolution X-ray structures that mimic the pre-catalytic state, the post-catalytic state and a competent state that can be transformed into the two other ones. Zn2+ (or Co2+) in Metal A site changes coordination from octahedral to tetrahedral after the chemical step, which explains the known higher affinity of Tdt for the primer strand when these ions are present, and metal A has to leave to allow the translocation of the primer strand and to clear the active site. Except for Zn2+, the sugar puckering of the primer strand 3' terminus changes from C2'-endo to C3'-endo during catalysis. Metal A is the last component that binds to the active site to complete its productive assembly
TdT in complex with dsDNA A5/T5G and ddCTP, to 2.80 A resolution, TdT in complex with dsDNA A5/T5GG and ddCTP to 1.99 A resolution and TdT in complex with ssDNA A5, dsDNA A5/T5GG-P, and ddCTP to 2.66 A resolution