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Literature summary for 2.7.7.31 extracted from
- Terminal deoxyribonucleotidyl transferase (1993), Methods Mol. Biol., 16, 95-105.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | use in production of synthetic homo- and heteropolymers, N-acetylation or N-alkylation of derivatives of dNTPs | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | - |
Homo sapiens |  |
| I- | - |
Homo sapiens | |
| NH4+ | - |
Homo sapiens | |
| phosphate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
oligonucleotide primers | - |
Homo sapiens | |
| 0.01 | - |
dATP | dGTP | Homo sapiens | |
| 0.5 | - |
dTTP | dCTP | Homo sapiens | |
| 1 | - |
homopolymer primers | - |
Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ | Homo sapiens | |
| Mg2+ | decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ | Homo sapiens | |
| Mg2+ | dGTP also optimally added in the presence of Mg2+ | Homo sapiens | |
| Mg2+ | polymerization of pyrimidines is best with Co2+ | Homo sapiens | |
| Zn2+ | in decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
- |
Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNA | Homo sapiens | involved in creating somatic diversity of immunoglobulin and T-cell receptor genes | diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
commercial preparation | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lymphocyte | enzyme activity is only found in the nuclei of pre-T and pre-B lymphocytes | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNA | involved in creating somatic diversity of immunoglobulin and T-cell receptor genes | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | primer required | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | reaction is not template directed | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
| dNTP + homopolymer primers | - |
Homo sapiens | ? | - |
? | |
| dNTP + oligonucleotide primers | - |
Homo sapiens | ? | - |
? | |
| dTTP + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, various organism | Homo sapiens |
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Release 2023.1 (January 2023)
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