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2.7.7.31: DNA nucleotidylexotransferase

This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.

Word Map on EC 2.7.7.31

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.31 DNA nucleotidylexotransferase

KM Value

KM Value on EC 2.7.7.31 - DNA nucleotidylexotransferase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00646 - 0.0176
2bA FAM oligo
-
0.000009
alpha-[2-N-(9-fluorenylmethoxycarbonyl)aminoethylphosphonyl]-beta,gamma-difluoromethylenediphosphonate
-
37°C, pH 7.2
0.000033
alpha-[2-N-(9-fluorenylmethoxycarbonyl)aminoethylphosphonyl]-beta,gamma-diphosphate
-
37°C, pH 7.2
0.1
D-beta-deoxythymidine-triphosphate
-
pH 6.6, 37°C, primer oligo(dT)20
0.004 - 0.01
dATP
0.071
dCTP
-
37°C, primer (dA)12-18
0.083 - 0.114
dGTP
0.00008 - 0.0002
DNA
0.000032 - 1.02
dTTP
1
homopolymer primers
-
-
-
0.02
oligo(dA)12-18
-
37°C, pH 8.3
0.001
oligonucleotide primers
-
-
-
0.0003 - 0.0025
poly(dA)50
additional information
additional information
-