Literature summary for 2.7.7.31 extracted from

Maezawa, S.; Nakano, S.; Kuniya, T.; Koiwai, O.; Koiwai, K.
Double-strand break repair based on short-homology regions is suppressed under terminal deoxynucleotidyltransferase expression, as revealed by a novel vector system for analysing DNA repair by nonhomologous end joining (2016), FEBS open bio, 6, 16-23.

Search for more literature for 2.7.7.31

Application

Application	Comment	Organism
analysis	construction of a nonhomologous end-joining assay vector NAV, containing mKate2, Venus and ccdB genes. Cotransfection of NAV with a construct expressing the restriction enzyme I-SceI generates a double-strand break in NAV that excises mKate2 and ccdB. Repair of this double-strand break produces an intact vector that expresses Venus, a green fluorescent protein. Cells bearing the repaired NAV lack the ccdB gene which slows cell proliferation, the cultures are enriched in cells containing repaired double-strand breaks	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
transfection of U2OS cell	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

General Information

General Information	Comment	Organism
physiological function	in cells overexpressing terminal deoxynucleotidyltransferase, the enzyme suppresses DNA repair that is based on short (one- or two-base) homology regions, to efficiently add deoxynucleotides during VDJ recombination in lymphoid cells	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)