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2.7.7.13: mannose-1-phosphate guanylyltransferase

This is an abbreviated version!
For detailed information about mannose-1-phosphate guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.13

Reaction

GTP
+
alpha-D-mannose 1-phosphate
=
diphosphate
 +
GDP-mannose

Synonyms

BceA, BceA(J) protein, BceAJ, CYT1, GDP-alpha-D-mannose pyrophosphorylase, GDP-D-mannose pyrophosphorylase, GDP-Man pyrophosphorylase, GDP-mannose pyrophosphorylase, GDP-mannose pyrophosphorylase B, GDP-ManPP, GDP-MP, GDPMan pyrophosphorylase, GMP, GMP3, GMPase, GMPBB, GMPP, GMPP1, GMPP2, GMPP3, GMPPB, GTP-alpha-D-Man-1-P guanylyltransferase, GTP-mannose 1-phosphate guanylyltransferase, GTP-mannose-1-phosphate guanylyltransferase, guanidine diphosphomannose pyrophosphorylase, guanidine diphosphomannose pyrophoyphorylase, guanosine 5'-diphosphate-mannose pyrophosphorylase, guanosine 5'-diphospho-D-mannose pyrophosphorylase, guanosine diphosphate mannose pyrophosphorylase, guanosine diphosphate mannose pyrophosphorylase B, guanosine diphosphate-mannose pyrophosphorylase, guanosine diphosphomannose pyrophosphorylase, guanosine triphosphate-mannose 1-phosphate guanylyltransferase, guanosine-diphospho-D-mannose pyrophosphorylase, guanylyltransferase, mannose 1-phosphate, LDBPK_230120, Man-1-PGTase, mannose 1-phosphate guanyltransferase, mannose 1-phosphate guanylyltransferase (guanosine triphosphate), mannose-1-phosphate guanyl transferase, mannose-1-phosphate guanyltransferase beta, mannose-1-phosphate guanylyltransferase, More, MPG, MPG transferase, multiple sugar-1-P NTase, NDP-sugar pyrophosphorylase, NSPase, Os01g0847200, Os03g0268400, OsVTC1-1, OsVTC1-3, OsVTC1-8, PFManC, PH0925, PH0925 protein, PIM-GMP, RfbM, VCT1-1, VCT1-3, VTC1, VTC1-1, VTC1-3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.13 mannose-1-phosphate guanylyltransferase

Cloned

Cloned on EC 2.7.7.13 - mannose-1-phosphate guanylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of cDNA
DNA and amino acid sequence determination and anaylsis, expression analysis and regulation of the expression, functional expression as GST-tagged enzyme in Escherichia coli strain BL21 under conditions suitable to reduce the formation of inclusion bodies using the vector pTgroE of the yeast Vig9 protein, overview
-
expressed in Escherichia coli BL21 Codon Plus (DE3)-RIPL strain
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and Arabidopsis thaliana
expression in Agrobacterium tumefaciens
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)pLysS
-
expression in of the His-tagged enzyme in Escherichia coli
expression of GST-fusion protein in Escherichia coli
expression of GST-GDPmannose pyrophosphorylase fusion protein in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli strain Origami (DE3) pLysS
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
-
expression of wild-type and several PMI-GMP mutants in algA mutant 8853
fused to glutathione S-transferase, expression in Escherichia coli, TOP10F'
-
gene Afsrb1, expression in Escherichia coli strain BL21(DE3)
gene bceA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene bceA, the gene is part of the Burkholderia cepacia IST408 exopolysaccharide biosynthetic cluster, physical organization, unrooted phylogenetic tree, DNA and amino acid sequence determination, analysis, and comparisons, overview. Expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GMP3, DNA and amino acid sequence determination and analysis, located on chromosome 3, quantitative real-time RT-PCR expression analysis,
gene MPG1, recombinant overexpression in Solanum lycopersicum ripe, fruits show enhanced L-ascorbate levels
gene OsVTC1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene OsVTC1-3, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene OsVTC1-8, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PH0925, recombinant expression of His-tagged enzyme PH0925 in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
genes OsMPG1, OsMPG2, OsMPG3 and OsMPG4, DNA and amino acid sequence, chromosomal locations, and alternative spliced forms determination and analysis, genetic organization, genomic distribution of MPG genes on rice chromosomes, and phylogenetic analysis, expression analysis, recombinant expression of GFP-tagged full-length MPG1 in Nicotiana tabacum using the Agrobacterium tumefaciens, strain LBA4404, transfection system. Functional complementation of yeast MPG1 YDL055C mutant by expression of OsMPG1, OsMPG2, OsMPG3 and OsMPG4 genes
-
His tagged version expressed in Escherichia coli BL21
His-tagged version expressed in Escherichia coli BL21(DE3)
-
overexpression in Escherichia coli
-
overexpression reveals changes in cell wall assembly, restores defects in O-glycosylation, but not those in N-glycosylation, that occur in Kluyveromyces lactis cells depleted for the hexokinase Rag5p, overexpression also enhances heterologous protein secretion in Kluyveromyces lactis
-
recombinant functional overexpression of Saccharomyces cerevisiae gene MPG1 in Solanum lycopersicum, fruits show 17-31fold enhanced enzyme activity and L-ascorbate levels
-