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Literature summary for 2.7.7.13 extracted from

  • Elling, L.; Ritter, J.E.; Verseck, S.
    Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose (1996), Glycobiology, 6, 591-597.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
GDP-mannose competitive vs. GTP, uncompetitive vs. mannose 1-phosphate Salmonella enterica
GDP-mannose
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
alpha-D-mannose 1-phosphate
-
Salmonella enterica
0.2
-
GTP
-
Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54045
-
2 * 54045, recombinant enzyme, deduced from nucleotide sequence Salmonella enterica
55000
-
2 * 55000, recombinant enzyme, SDS-PAGE Salmonella enterica
108000
-
recombinant enzyme, gel filtration Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate Arthrobacter sp.
-
GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate Salmonella enterica
-
GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Salmonella enterica
-
group B
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, Q-Sepharose FF, Phenyl-Sepharose, diafiltration, Sephadex G 75, Sephadex G 200, ammonium sulfate Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.75
-
recombinant enzyme Salmonella enterica

Storage Stability

Storage Stability Organism
4°C, 25% glycerol, 47 d, 25% loss of activity Salmonella enterica
4°C, 3 M ammonium sulfate, 47 d, 40% loss of activity, 122 d, 50% loss of activity Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate
-
Sus scrofa GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Arthrobacter sp. GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Salmonella enterica GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Salmonella enterica diphosphate + GDP-mannose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, recombinant enzyme, SDS-PAGE Salmonella enterica
dimer 2 * 54045, recombinant enzyme, deduced from nucleotide sequence Salmonella enterica

Synonyms

Synonyms Comment Organism
GDP-alpha-D-mannose pyrophosphorylase
-
Salmonella enterica
RfbM enzyme is encoded by the rfbM gene Salmonella enterica
RfbM
-
Salmonella enterica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
GDP-mannose competitive vs. GTP Salmonella enterica
0.12
-
GDP-mannose uncompetitive vs. mannose 1-phosphate Salmonella enterica