Cloned (Comment) | Organism |
---|---|
His tagged version expressed in Escherichia coli BL21 | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-6-deoyxmannose | - |
Trypanosoma brucei | |
GDP-mannose | feedback inhibition | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
D-mannose 1-phosphate | - |
Trypanosoma brucei | |
0.067 | - |
GTP | - |
Trypanosoma brucei | |
0.29 | - |
ATP | - |
Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in bloodstream-form | Trypanosoma brucei | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for divalent cations, partially replaced by Mn2+ and Cu2+ | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | B9WNA1 | - |
- |
Purification (Comment) | Organism |
---|---|
batch absorption to nickel-agarose | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
- |
Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-mannose 1-phosphate | - |
Trypanosoma brucei | diphosphate + ADP-mannose | - |
? | |
GTP + D-mannose 1-phosphate | - |
Trypanosoma brucei | diphosphate + GDP-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
guanidine diphosphomannose pyrophosphorylase | - |
Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
neutral pH optimum | Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
GDP-mannose | competitive inhibitor with respect to GTP | Trypanosoma brucei | |
0.002 | - |
GDP-mannose | mixed-type inhibition with respect to mannose 1-phosphate | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 values for ATP and CTP above 0.625 mM | Trypanosoma brucei | additional information | |
0.008 | - |
feedback inhibition | Trypanosoma brucei | GDP-mannose | |
0.013 | - |
- |
Trypanosoma brucei | GDP-6-deoxymannose |
General Information | Comment | Organism |
---|---|---|
physiological function | essential in bloodstream-form | Trypanosoma brucei |