Application | Comment | Organism |
---|---|---|
agriculture | over-expression of isoform Gmp3 increases total L-ascorbic acid contents and enhances the tolerance to oxidative stress in tomato. Knock-down of Gmp3 significantly decreases L-ascorbic acid contents below the threshold level and alters the phenotype of tomato plants with lesions and further senescence | Solanum tuberosum |
Cloned (Comment) | Organism |
---|---|
gene GMP3, DNA and amino acid sequence determination and analysis, located on chromosome 3, quantitative real-time RT-PCR expression analysis, | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of GMP3 overexpressing and RNAi knockdown lines | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Solanum lycopersicum | - |
diphosphate + GDP-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q54K39 | SGN-U568547 | - |
Solanum tuberosum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | high expression level | Solanum lycopersicum | - |
flower | high level of transcripts | Solanum tuberosum | - |
fruit | - |
Solanum lycopersicum | - |
fruit | low level of transcripts | Solanum tuberosum | - |
leaf | - |
Solanum lycopersicum | - |
leaf | young leaf, high level of transcripts | Solanum tuberosum | - |
additional information | SlGMP3 transcript levels are high in stems, flowers, and young leaves, whereas low in roots, and fruits at breaker and red ripe stages, expression patterns, overview | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
root | low level of transcripts | Solanum tuberosum | - |
stem | - |
Solanum lycopersicum | - |
stem | high level of transcripts | Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Solanum tuberosum | diphosphate + GDP-mannose | - |
? | |
GTP + alpha-D-mannose 1-phosphate | - |
Solanum lycopersicum | diphosphate + GDP-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDP-D-mannose pyrophosphorylase | - |
Solanum tuberosum |
GDP-D-mannose pyrophosphorylase | - |
Solanum lycopersicum |
GMP | - |
Solanum lycopersicum |
GMP3 | - |
Solanum tuberosum |
General Information | Comment | Organism |
---|---|---|
evolution | four members of GMP gene family are encoded in the tomato genome, which constitutively expressed in various tissues in distinct expression patterns | Solanum lycopersicum |
malfunction | reducing ascorbic acid leads to leaf lesion and defence response in knock-down of the ascorbic acid biosynthetic enzyme GDP-D-mannose pyrophosphorylase gene in tomato plant. Constitutive overexpression of SlGMP3 increases the total ascorbic acid contents in the fully expanded leaves and red ripe fruits, with enhanced tolerance to oxidative stress in SlGMP3 overexpressing plants. In SlGMP2/3-knockdown lines, the total ascorbic acid contents in leaves, immature green fruits, and breaker fruits decrease dramatically. With the developments of fruit, the ascorbic acid levels increase up to 46% in breaker stage compared with immature green stage in wild-type plants, whereas no major difference is observed between two stages of SlGMP2/3-knockdown lines. In SlGMP2/3-knockdown transgenic line KD17, SlGME2 and SlGGP1 are significantly up-regulated (over one fold) compared with the wild-type plants. The spontaneous lesions on the leaves of SlGMP2/3-knockdown plants develop in a similar manner as a hypersensitive response after pathogen attack. Impairment of the photosynthetic system in SlGMP2/3-knockdown plants, overview | Solanum lycopersicum |
metabolism | GDP-mannose pyrophosphorylase which catalyzes the conversion of D-mannose-1-P to GDP-D-mannose in AsA biosynthetic pathway. GDP-D-mannose pyrophosphorylase is a vital enzyme in the Smirnoff-Wheeler's pathway for the biosynthesis of ascorbic acid in plants | Solanum lycopersicum |
physiological function | over-expression of isoform Gmp3 increases total L-ascorbic acid contents and enhances the tolerance to oxidative stress in tomato. Knock-down of Gmp3 significantly decreases L-ascorbic acid contents below the threshold level and alters the phenotype of tomato plants with lesions and further senescence. This symptom could result from failing to instantly deplete the reactive oxygen species as decline of free radical scavenging activity. More reactive oxygen species accumulate in the leaves and then trigger expressions of defence-related genes. Symptoms occur on the leaves similar to hypersensitive responses against pathogens | Solanum tuberosum |